School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
Mol Genet Genomics. 2022 Jul;297(4):935-945. doi: 10.1007/s00438-022-01883-5. Epub 2022 May 6.
In the context of widespread bacterial contamination and the endless emergence of antibiotic-resistant bacteria, more effective ways to control pathogen infection are urgently needed. Phages become potential bactericidal agents due to their bactericidal specificity and not easy resistance to bacteria. But an important factor limiting its development is the lack of phage species. Therefore, the isolation of more new phages and studying their biological and genomic characteristics is of great significance for subsequent applications. So, in this study, SGF3, a Microviridae phage, which has shown lytic activity against Shigella flexneri, was isolated, purified, and characterized. Morphological and phylogenetic analyses identified it as a phiX174 species belonging to the Microviridae family. The latent period of phage SGF3 was 20 min, with an average burst size of approximately 7.1. Host spectrum experiments indicated its strong host specificity. Furthermore, the biofilm removal efficiency was increased by 20%-25% when SGF3 was coupled with other phages. In conclusion, the phage SGF3 found in this study was a lytic phage belonging to the Microviral family, and could be added as an auxiliary material in the phage cocktail. Studies of its characteristics and bactericidal properties had enriched the germplasm resources of microphages, provided more potential material in fighting against emerging and existing multidrug-resistant bacteria.
在广泛的细菌污染和抗生素耐药菌不断出现的情况下，急需更有效的方法来控制病原体感染。噬菌体由于其杀菌特异性和不易产生细菌耐药性而成为潜在的杀菌剂。但限制其发展的一个重要因素是噬菌体种类的缺乏。因此，分离更多新的噬菌体并研究其生物学和基因组特征对于后续的应用具有重要意义。因此，在这项研究中，分离、纯化并鉴定了一种对福氏志贺菌具有裂解活性的微病毒科噬菌体 SGF3。形态学和系统发育分析将其鉴定为属于微病毒科的 phiX174 种。噬菌体 SGF3 的潜伏期为 20 分钟，平均爆发量约为 7.1。宿主谱实验表明其具有很强的宿主特异性。此外，当 SGF3 与其他噬菌体结合使用时，生物膜去除效率提高了 20%-25%。综上所述，本研究中发现的噬菌体 SGF3 是一种属于微病毒科的裂解性噬菌体，可以作为噬菌体鸡尾酒的辅助材料添加。对其特性和杀菌性能的研究丰富了微噬菌体的种质资源，为应对新兴和现有多药耐药菌提供了更多潜在的物质。
