大肠杆菌中核糖核苷二磷酸还原酶合成的调控：该酶的比活性与DNA复制扰动的关系
Regulation of the synthesis of ribonucleoside diphosphate reductase in Escherichia coli: specific activity of the enzyme in relationship to perturbations of DNA replication.
作者信息
Filpula D, Fuchs J A
出版信息
J Bacteriol. 1978 Aug;135(2):429-35. doi: 10.1128/jb.135.2.429-435.1978.
Ribonucleoside diphosphate reductase (RDP reductase) activity was found to greatly increase after a shift to the nonpermissive temperature in Escherichia coli mutants temperature sensitive for DNA elongation (dnaE dnaG dnaZ lig) or DNA initiation (dnaA dnaC dnaI). However, the kinetics of increase in RDP reductase after a shift to nonpermissive conditions were significantly different in initiation-defective mutants compared with elongation-defective mutants. In strains without defects in DNA metabolism, the specific activity of RDP reductase was found to increase with increasing growth rate. Nutritional shifts to faster growth conditions caused cells to transiently overproduce RDP reductase before adjusting to the new steady-state conditions.
在对DNA延伸（dnaE、dnaG、dnaZ、lig）或DNA起始（dnaA、dnaC、dnaI）温度敏感的大肠杆菌突变体中，当转移到非允许温度后，发现核糖核苷二磷酸还原酶（RDP还原酶）活性大幅增加。然而，与延伸缺陷型突变体相比，起始缺陷型突变体在转移到非允许条件后，RDP还原酶活性增加的动力学有显著差异。在DNA代谢无缺陷的菌株中，发现RDP还原酶的比活性随生长速率的增加而增加。营养转移到更快的生长条件会使细胞在适应新的稳态条件之前短暂地过量产生RDP还原酶。
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