Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Department of Agriculture, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Plant Dis. 2022 Dec;106(12):3178-3186. doi: 10.1094/PDIS-12-21-2793-RE. Epub 2022 Nov 6.
Soybean ( L.) seeds showing serious symptoms from rotted pods were collected from fields during the harvesting period (July to August 2020) in Taihu Farm, Jingzhou City, Hubei Province, China. strains were frequently encountered during fungal isolation. According to the morphology and prepathogenicity tests, six strains showing variable effects on the seeds were selected for identification based on morphology and multilocus phylogenetic analysis of the internal transcribed spacer (ITS) region of the ribosomal DNA, translation elongation factor (α), calmodulin (), β-tubulin (), and partial RNA polymerase second largest subunit (), and to evaluate the pathogenic abilities on seed, root, and pod. The results indicated that the strains contained two species ( and ) in the species complex (FFSC) and two species ( and ) from the species complex (FIESC). The two species of FFSC were more aggressive than those of FIESC on soybean seed, root, and pod. Among the strains, YZU 201408 exhibited the most pathogenicity on all tests, with 72.2 to 90% disease severity.
从中国湖北省荆州市太湖农场的收获期（2020 年 7 月至 8 月）的腐烂豆荚中收集到出现严重症状的大豆（ L.）种子。在真菌分离过程中经常遇到 菌株。根据形态学和预致病性测试，选择了六个对种子有不同影响的菌株，根据核糖体 DNA 的内部转录间隔区（ITS）区域、翻译延伸因子（α）、钙调蛋白（）、β-微管蛋白（）和部分 RNA 聚合酶第二大亚基（）的形态和多基因座系统发育分析进行鉴定，并评估对种子、根和荚的致病能力。结果表明，这些菌株在 种复合种（FFSC）中包含两个种（和），在 种复合种（FIESC）中包含两个种（和）。FFSC 的两个种在大豆种子、根和荚上比 FIESC 的种更具攻击性。在这些菌株中，YZU 201408 号在所有测试中表现出最强的致病性，病情严重程度为 72.2%至 90%。
