College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, China.
College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, China.
Poult Sci. 2022 Jul;101(7):101916. doi: 10.1016/j.psj.2022.101916. Epub 2022 Apr 9.
Cecal epithelial cell damage is a key factor in host injure during the development of E. tenella. The intracellular free Ca of the host cell is closely related to the invasion, development and proliferation of intracellular parasites, and cell damage. To determine the relationship between Ca and host cell damage in the schizogenic stage of E. tenella, we established a chick embryo cecal epithelial cells model of E. tenella infection. Fluorescence staining, flow cytometry, transmission electron microscopy, inhibition and blocking experiments were used to detect the damage effect and mechanism of host cells during the schizogenic stage of E. tenella. The results showed that the host cells cytoskeletal remodeling, cell and organelle structure was destroyed, and apoptosis and necrosis were increased during the schizont stage of E. tenella. Furthermore, the above-mentioned effects of the schizogenic stage of E. tenella on cells can be alleviated by reducing the intracellular Ca concentration in the host cells. These observations indicate that the effect of host cell injury was closely related to Ca during schizont stage of E. tenella.
空肠上皮细胞损伤是鸡球虫发育过程中宿主损伤的关键因素。宿主细胞内的游离钙与细胞内寄生虫的入侵、发育和增殖以及细胞损伤密切相关。为了确定柔嫩艾美耳球虫裂殖阶段的钙与宿主细胞损伤之间的关系，我们建立了柔嫩艾美耳球虫感染鸡胚空肠上皮细胞模型。通过荧光染色、流式细胞术、透射电镜、抑制和阻断实验，检测了柔嫩艾美耳球虫裂殖阶段对宿主细胞的损伤作用及其机制。结果表明，在柔嫩艾美耳球虫裂殖阶段，宿主细胞的细胞骨架重构，细胞和细胞器结构被破坏，细胞凋亡和坏死增加。此外，通过降低宿主细胞内的细胞内钙浓度，可以减轻柔嫩艾美耳球虫裂殖阶段对细胞的上述作用。这些观察结果表明，在柔嫩艾美耳球虫裂殖阶段，宿主细胞损伤的效果与钙密切相关。
