Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China.
College of Marine Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Fish Shellfish Immunol. 2022 Jun;125:128-140. doi: 10.1016/j.fsi.2022.04.045. Epub 2022 May 3.
Traditionally, invertebrates were thought to lack immune memory owing to a lack of acquired immune-related factors such as immunoglobulin. Nonetheless, with the in-depth consideration of invertebrate immune priming, scholars have gradually realized that the immune defenses of invertebrates are more complex than previously imagined. In the current investigation, the survival rate of Vibrio parahaemolyticus re-infected Haliotis discus hannai (VV group) was significantly different from the other groups (p < 0.05), indicating that an enhanced immune response may commence after first exposure to the same strain of V. parahaemolyticus. The transcriptome profiles of hemocytes obtained 102,052 unigenes, and 27,449 of them were annotated successfully. Venn diagram analysis showed that 2832 DEGs commonly responded to the first and second immune responses. 1734 "immune response genes" and 1460 "potential immune-enhancing genes" were also identified. A comparison of both "immune response genes" and "potential immune-enhancing genes" revealed 1019 immune-enhancing regulatory genes and 281 essential immune-enhancing genes. According to the KEGG enrichment analysis results of ERGs and EEGs, classical immune-related signaling pathways, such as NF-kappa B signaling pathway, NOD-like receptor signaling pathway, IL-17 signaling pathway, and TLR signaling pathway were significantly enriched, indicating that they were all involved in the response to V. parahaemolyticus re-infection and were likely dominant in the immune enhancement process of H. discus hannai hemocytes. The intermolecular interactions generated by Cytoscape after re-infection of V. parahaemolyticus appear more intuitively to demonstrate that hemocytes regulation was not an independent process, but rather an intricate regulatory network. H. discus hannai demonstrated enhanced immunological activity after re-infection with V. parahaemolyticus, showing immune memory in hemocytes. The current study's findings have broadened the study of immune enhancement in invertebrates and laid the framework for future research into the molecular mechanism of immune enhancement in abalones.
传统上，由于缺乏免疫球蛋白等获得性免疫相关因子，人们认为无脊椎动物缺乏免疫记忆。然而，随着对无脊椎动物免疫启动的深入研究，学者们逐渐认识到无脊椎动物的免疫防御比以前想象的更为复杂。在本次研究中，感染过副溶血弧菌的杂色鲍（VV 组）再感染副溶血弧菌的存活率与其他组有显著差异（p<0.05），这表明同种副溶血弧菌首次感染后可能会引发增强的免疫反应。血细胞的转录组图谱获得了 102052 条 unigenes，其中 27449 条成功注释。Venn 图分析显示，2832 个 DEGs 共同对首次和第二次免疫反应做出响应。还鉴定出 1734 个“免疫反应基因”和 1460 个“潜在免疫增强基因”。对这两种“免疫反应基因”和“潜在免疫增强基因”进行比较，发现了 1019 个免疫增强调控基因和 281 个必需免疫增强基因。根据 ERGs 和 EEGs 的 KEGG 富集分析结果，经典的免疫相关信号通路，如 NF-kappa B 信号通路、NOD 样受体信号通路、IL-17 信号通路和 TLR 信号通路，均显著富集，这表明它们均参与了副溶血弧菌再感染的反应，并且可能在杂色鲍血细胞的免疫增强过程中占主导地位。副溶血弧菌再感染后 Cytoscape 生成的分子间相互作用更直观地表明，血细胞的调控不是一个独立的过程，而是一个复杂的调控网络。杂色鲍在副溶血弧菌再感染后表现出增强的免疫活性，血细胞表现出免疫记忆。本研究拓宽了无脊椎动物免疫增强研究的范围，为今后研究鲍鱼的免疫增强分子机制奠定了框架。
