School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, South China University of Technology, Guangzhou 510641, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, South China University of Technology, Guangzhou 510641, China; Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, China.
Int J Biol Macromol. 2022 Jun 15;210:518-529. doi: 10.1016/j.ijbiomac.2022.04.206. Epub 2022 May 6.
In this study, Moringa oleifera leaf (MOL) flavonoids (MOLF) with strong α-glucosidase inhibitory activity and MOL polysaccharides (MOLP) with strong cholic acid-binding capacity were efficiently prepared by two-stage extraction method and mixed in a certain proportion for development of MOL highly-processed products with hypoglycemic and hypolipemic potentials. Quercetin-3-O-glucoside (6.86%) and kaempferol-3-O-glucoside (4.02%) were identified as the main components of MOLF. MOLP constructed by galactose, arabinose, rhamnose and galacturonic acid possessed the strongest effects on delaying glucose diffusion and dialysis, delaying starch digestion, binding bile acids and inhibiting cholesterol micelle solubility, being the best MOL highly-processed products for regulating carbohydrate and lipid digestion and absorption. MOLF and MOLP had synergistic effect on delaying glucose diffusion and dialysis, delaying starch digestion and binding bile acids, while MOLF impaired the inhibitory effect of MOLP on cholesterol micelle solubility. Compared with MOL primary-processed products including MOL powder and de-phenolic MOL powder, MOL highly-processed products including MOLP and MOLF-MOLP complex possessed stronger hypoglycemic/hypolipemic potentials.
在这项研究中，通过两段式提取法高效制备具有强α-葡萄糖苷酶抑制活性的辣木叶（MOL）类黄酮（MOLF）和具有强胆酸结合能力的 MOL 多糖（MOLP），并按一定比例混合，开发具有降血糖和降血脂潜力的 MOL 深加工产品。鉴定出 MOLF 的主要成分为槲皮素-3-O-葡萄糖苷（6.86%）和山奈酚-3-O-葡萄糖苷（4.02%）。由半乳糖、阿拉伯糖、鼠李糖和半乳糖醛酸构成的 MOLP 对延缓葡萄糖扩散和透析、延缓淀粉消化、结合胆汁酸和抑制胆固醇胶束溶解度的效果最佳，是调节碳水化合物和脂质消化吸收的最佳 MOL 深加工产品。MOLF 和 MOLP 对延缓葡萄糖扩散和透析、延缓淀粉消化和结合胆汁酸具有协同作用，而 MOLF 则削弱了 MOLP 对胆固醇胶束溶解度的抑制作用。与 MOL 初级加工产品（包括 MOL 粉和去酚 MOL 粉）相比，MOL 深加工产品（包括 MOLP 和 MOLF-MOLP 复合物）具有更强的降血糖/降血脂潜力。
