在中间温度下培养的大肠杆菌热敏性dnaA突变体的细胞直径。
Diameter of cells of a thermosensitive dnaA mutant of Escherichia coli cultivated at intermediate temperatures.
作者信息
Pritchard R H, Meacock P A, Orr E
出版信息
J Bacteriol. 1978 Aug;135(2):575-80. doi: 10.1128/jb.135.2.575-580.1978.
Abstract
Strains of Escherichia coli K-12 carrying the dnaA46 mutation exhibited a progressively decreasing DNA concentration and a progressively increasing cell size as the temperature was raised from 31 to 37 degrees C. Above 37 degrees C, steady-state exponential growth could not be maintained. The increase in average cell size with increasing growth temperature was due to an increase in cell length. There was no change in cell width. This seems to rule out the hypothesis that the increase in cell width in thy strains cultivated on low concentrations of thymine is due to the decrease in DNA concentration which also occurs under these conditions.
摘要
携带dnaA46突变的大肠杆菌K-12菌株，当温度从31℃升高到37℃时，其DNA浓度逐渐降低，细胞大小逐渐增加。在37℃以上，无法维持稳态指数生长。随着生长温度升高，平均细胞大小的增加是由于细胞长度增加。细胞宽度没有变化。这似乎排除了这样一种假说，即在低浓度胸腺嘧啶上培养的thy菌株中细胞宽度的增加是由于在这些条件下也会发生的DNA浓度降低。
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