Chen Xiang, Mao Yucheng, Chai Weiguo, Yan Kaijing, Liang Zongsuo, Xia Pengguo
Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou, 310024, Zhejiang Province, China.
Protoplasma. 2023 Jan;260(1):189-205. doi: 10.1007/s00709-022-01770-1. Epub 2022 May 7.
The myeloblastosis (MYB) gene family, involved in regulating many important physiological and biochemical processes, is one of the largest transcript factor superfamilies in plants. Since the identification of genome sequencing of Panax notoginseng has been completed, there was little known about the whole genome of its specific MYB gene family and the response to abiotic stresses, in consideration of the excessive application of nitrogen fertilizers in P. notoginseng. In this study, 123 PnMYB genes (MYB genes of P. notoginseng) have been identified and divided into 3 subfamilies by the phylogenetic analysis. These PnMYB genes were unevenly located on 12 chromosomes. Meanwhile, the gene structure and protein conserved domain were established by MEME Suite. The analysis of collinear relationships reflected that there were 121 homologous genes between P. notoginseng and Arabidopsis and 30 between P. notoginseng and rice. Moreover, cis-acting elements of PnMYB gene promoters were predicted which indicated that PnMYBs are involved in biotic, abiotic stress, and hormone induction. The expressions of PnMYB transcription factors in its roots, flowers, and leaves were detected by qRT-PCR and they had tissue-specific expressions and related to the growth of different tissues. Under nitrogen stress, MYB transcription factors had great feedback. Ten R2R3-MYB subfamily genes were significantly induced and indicated the possible function of protecting P. notoginseng from excess nitrogen. With further knowledge on identification of PnMYB gene related to tissue selectivity and abiotic stresses, this study laid the foundation for the functional development of PnMYB gene family and improved the cultivation of P. notoginseng.
成髓细胞瘤（MYB）基因家族参与调控许多重要的生理和生化过程，是植物中最大的转录因子超家族之一。鉴于三七中氮肥的过度施用，自三七基因组测序完成鉴定以来，对其特定MYB基因家族的全基因组及其对非生物胁迫的响应了解甚少。在本研究中，已鉴定出123个三七MYB基因（PnMYB基因），并通过系统发育分析将其分为3个亚家族。这些PnMYB基因不均匀地分布在12条染色体上。同时，利用MEME Suite确定了基因结构和蛋白质保守结构域。共线性关系分析表明，三七与拟南芥之间有121个同源基因，三七与水稻之间有30个同源基因。此外，对PnMYB基因启动子的顺式作用元件进行了预测，表明PnMYB参与生物、非生物胁迫和激素诱导。通过qRT-PCR检测了PnMYB转录因子在其根、花和叶中的表达，它们具有组织特异性表达，且与不同组织的生长相关。在氮胁迫下，MYB转录因子有很大的反馈。10个R2R3-MYB亚家族基因被显著诱导，表明其可能具有保护三七免受过量氮危害的功能。随着对与组织选择性和非生物胁迫相关的PnMYB基因鉴定的进一步了解，本研究为PnMYB基因家族的功能开发奠定了基础，并改善了三七的栽培。
