College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Institute of Agriculture, The University of Western Australia, Crawley, WA 6009, Australia.
Int J Mol Sci. 2024 Jun 8;25(12):6355. doi: 10.3390/ijms25126355.
TGA transcription factors belong to Group D of the bZIP transcription factors family and play vital roles in the stress response of plants. is an oil crop with rich economic value. However, a systematic analysis of gene family members in has not yet been reported. In this study, we identified 39 full-length genes in , renamed ~. Thirty-nine genes were distributed on 18 chromosomes, mainly located in the nucleus, and differences were observed in their 3D structures. Phylogenetic analysis showed that 39 genes could be divided into five groups. The genes in the same group had similar structure and motif compositions, and all the genes had the same conserved bZIP and DOG1 domains. Phylogenetic and synteny analysis showed that the genes had a close genetic relationship with the genes of the , and and may play an important role in evolution. In addition, qRT-PCR revealed that three genes () showed significant changes in eight experimental materials after drought treatment. Meanwhile, it can be inferred from the results of drought treatment on different varieties of rapeseed that the stress tolerance of parental rapeseed can be transmitted to the offspring through hybridization. In short, these findings have promoted the understanding of the gene family and will contribute to future research aimed at resistant breeding.
TGA 转录因子属于 bZIP 转录因子家族的 D 组，在植物的应激反应中发挥着重要作用。 是一种具有丰富经济价值的油料作物。然而，对 基因家族成员的系统分析尚未见报道。在本研究中，我们在 中鉴定了 39 个全长 基因，重新命名为~。39 个 基因分布在 18 条染色体上，主要位于核内，其 3D 结构存在差异。系统发育分析表明，39 个 基因可分为 5 组。同一组的 基因具有相似的结构和基序组成，所有 基因都具有相同的保守 bZIP 和 DOG1 结构域。系统发育和共线性分析表明， 基因与 的 基因具有密切的遗传关系， 和 可能在进化中发挥重要作用。此外，qRT-PCR 显示，在 8 种实验材料干旱处理后，三个基因（）发生了显著变化。同时，从不同油菜品种干旱处理的结果可以推断，亲本油菜的耐胁迫性可以通过杂交传递给后代。总之，这些发现促进了对 基因家族的理解，并将有助于未来的 抗性育种研究。
