Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
Tianjin TASLY Modern Chinese Medicine Resources Co., Ltd., Tianjin, 300402, People's Republic of China.
Biochem Genet. 2024 Aug;62(4):2631-2651. doi: 10.1007/s10528-023-10567-7. Epub 2023 Nov 24.
The dehydration response element-binding (DREB) transcription factor is a subfamily of AP2/ERF. It actively responds to various abiotic stresses in plants. As one of the representative plants, Panax notoginseng is sensitive to Nitrogen (N). Here, bioinformatics analysis, the identification, chromosomal location, phylogeny, structure, cis-acting elements, and collinearity of PnDREBs were analyzed. In addition, the expression levels of PnDREBs were analyzed by quantitative reverse transcription PCR. In this study, 54 PnDREBs were identified and defined as PnDREB1 to PnDREB54. They were divided into 6 subfamilies (A1-A6). And 44 PnDREBs were irregularly distributed on 10 of 12 chromosomes. Each group showed specific motifs and exon-intron structures. By predicting cis-acting elements, the PnDREBs may participate in biotic stress, abiotic stress, and hormone induction. Collinear analysis showed that fragment duplication events were beneficial to the amplification and evolution of PnDREB members. The expression of PnDREBs showed obvious tissue specificity in its roots, flowers, and leaves. In addition, under the action of ammonium nitrogen and nitrate nitrogen at the 15 mM level, the level of PnDREB genes expression in roots varied to different degrees. In this study, we identified and characterized PnDREBs for the first time, and analyzed that PnDREBs may be related to the response of P. Notoginseng to N sensitiveness. The results of this study lay a foundation for further research on the function of PnDREBs in P. Notoginseng.
脱水应答元件结合（DREB）转录因子是 AP2/ERF 的一个亚家族。它在植物中积极响应各种非生物胁迫。作为代表性植物之一，三七对氮（N）敏感。在这里，通过生物信息学分析，鉴定、染色体定位、系统发育、结构、顺式作用元件和共线性分析了 PnDREBs。此外，通过定量逆转录 PCR 分析了 PnDREB 的表达水平。在这项研究中，鉴定了 54 个 PnDREB，并将其定义为 PnDREB1 至 PnDREB54。它们分为 6 个亚家族（A1-A6）。44 个 PnDREB 不规则地分布在 12 条染色体中的 10 条上。每组显示出特定的基序和外显子-内含子结构。通过预测顺式作用元件，PnDREB 可能参与生物胁迫、非生物胁迫和激素诱导。共线性分析表明，片段重复事件有利于 PnDREB 成员的扩增和进化。PnDREB 在其根、花和叶中的表达表现出明显的组织特异性。此外，在 15mM 水平的铵氮和硝酸盐氮作用下，根中 PnDREB 基因表达水平的变化程度不同。在这项研究中，我们首次鉴定和表征了 PnDREB，并分析了 PnDREB 可能与三七对 N 敏感性的反应有关。本研究结果为进一步研究 PnDREB 在三七中的功能奠定了基础。
