School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Zhejiang Institute of Product Quality and Safety Science, Hangzhou 310018, China.
Colloids Surf B Biointerfaces. 2022 Aug;216:112528. doi: 10.1016/j.colsurfb.2022.112528. Epub 2022 May 2.
Macrophages polarization in bone immune microenvironment is crucial in bone regeneration. In this work, mineralized collagen (MC) coatings with photo-thermal effect were prepared through incorporation of polydopamine (PDA). MC coatings with different thicknesses were deposited on titanium substrate through electrochemical deposition. PDA preformed on the substrate, acting as a photo-thermal agent. The effects of light illumination, i.e., different thermal effects, on the polarization of mouse bone marrow-derived macrophages were explored. It was found that heat can promote the M1 polarization of macrophages and inhibit the M2 polarization. Also, gene expression results revealed that such photo illumination based macrophage modulation is effective and safe. It provides a possible way for the design of functional materials to regulate the bone immune microenvironment.
在骨免疫微环境中，巨噬细胞的极化在骨再生中至关重要。在这项工作中，通过掺入聚多巴胺（PDA）制备了具有光热效应的矿化胶原（MC）涂层。通过电化学沉积在钛基底上沉积了不同厚度的 MC 涂层。基底上预先形成的 PDA 作为光热剂。研究了光照，即不同的热效应，对小鼠骨髓来源的巨噬细胞极化的影响。结果发现，热量可以促进巨噬细胞的 M1 极化，并抑制 M2 极化。此外，基因表达结果表明，这种基于光照射的巨噬细胞调节是有效和安全的。它为设计调节骨免疫微环境的功能材料提供了一种可能的方法。
