通过免疫荧光法对盘基网柄菌变形虫中的肌动蛋白进行定位。
Localization of actin in Dictyostelium amebas by immunofluorescence.
作者信息
Eckert B S, Lazarides E
出版信息
J Cell Biol. 1978 Jun;77(3):714-21. doi: 10.1083/jcb.77.3.714.
Abstract
Antibody prepared against avian smooth muscle actin has been used to localize actin in the slime mold, Dictyostelium discoideum. The distribution of actin in migrating cells is different from that in feeding cells. Migrating amebas display fluorescence primarily in advancing regions whereas feeding amebas show uniform fluorescence throughout. The reaction is specific for actin since the fluorescence observed is blocked when the antibody is absorbed by actin purified from avian skeletal muscle, human platelets, and Dictyostelium. These results, in addition to describing the distribution of actin in D. discoideum, demonstrate that actins from these diverse sources share at least one common antigenic determinant.
摘要
针对禽平滑肌肌动蛋白制备的抗体已用于在黏菌盘基网柄菌中定位肌动蛋白。迁移细胞中肌动蛋白的分布与摄食细胞中的不同。迁移的变形虫主要在前进区域显示荧光，而摄食的变形虫则在整个细胞中显示均匀的荧光。该反应对肌动蛋白具有特异性，因为当抗体被从禽骨骼肌、人血小板和盘基网柄菌中纯化的肌动蛋白吸收时，观察到的荧光会被阻断。这些结果除了描述盘基网柄菌中肌动蛋白的分布外，还表明来自这些不同来源的肌动蛋白至少共享一个共同的抗原决定簇。
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