Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2022 Jul;49(7):6085-6091. doi: 10.1007/s11033-022-07397-y. Epub 2022 May 8.
Celiac disease (CeD) and inflammatory bowel disease (IBD) are accompanied by impaired immune responses. To study the immune regulation of these diseases, we evaluated the expression levels of pro-inflammatory (IL-8 and IL-17 A) and anti-inflammatory (IL-10) cytokines in intestinal biopsy specimens of CeD and IBD patients in comparison to healthy subjects.
Intestinal biopsies were collected from 33 patients with IBD, 47 patients with CeD, and 20 healthy individuals. Total RNA was extracted and mRNA expression levels of IL-8, IL-17 A and IL-10 were assessed by qPCR. P-value < 0.05 was considered statistically significant. The expression levels of IL-8 and IL-17 A were higher in biopsies of IBD (UC and CD) and CeD patients compared to the control group (P < 0.05). IBD patients (UC and CD) had higher IL-8 intestinal level than CeD patients (P < 0.0001 and P = 0.0007, respectively). The expression of IL-10 was significantly down-regulated in intestinal biopsies of CeD and IBD patients compared with controls (P < 0.001). In addition, the expression level of this cytokine was significantly lower in IBD patients (P < 0.001 for UC patients and P < 0.0001 for CD patients) than CeD group.
The three selected pro- and anti-inflammatory cytokines showed a similar expression pattern in both IBD and CeD patients. As IBD and CeD are immune-mediated disorders and are accompanied by inflammatory events, the understanding of the similarities and differences among them can help researchers to find out useful candidate therapeutic protocols. We suggest that larger cohort studies be organized to achieve more insights into this regulation.
乳糜泻（CeD）和炎症性肠病（IBD）伴有免疫反应受损。为了研究这些疾病的免疫调节，我们评估了 CeD 和 IBD 患者与健康受试者相比，肠道活检标本中促炎（IL-8 和 IL-17A）和抗炎（IL-10）细胞因子的表达水平。
从 33 例 IBD 患者、47 例 CeD 患者和 20 名健康个体中采集肠道活检。提取总 RNA，通过 qPCR 评估 IL-8、IL-17A 和 IL-10 的 mRNA 表达水平。P 值<0.05 被认为具有统计学意义。与对照组相比，IBD（UC 和 CD）和 CeD 患者的活检中 IL-8 和 IL-17A 的表达水平更高（P<0.05）。与 CeD 患者相比，IBD 患者（UC 和 CD）的肠道 IL-8 水平更高（分别为 P<0.0001 和 P=0.0007）。与对照组相比，CeD 和 IBD 患者的肠道活检中 IL-10 的表达明显下调（P<0.001）。此外，与 CeD 组相比，IBD 患者（UC 患者为 P<0.001，CD 患者为 P<0.0001）的这种细胞因子表达水平显著降低。
三种选定的促炎和抗炎细胞因子在 IBD 和 CeD 患者中表现出相似的表达模式。由于 IBD 和 CeD 是免疫介导的疾病，伴有炎症事件，因此了解它们之间的相似性和差异可以帮助研究人员找到有用的候选治疗方案。我们建议组织更大的队列研究，以更深入地了解这种调节。
