Department of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Department of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121286. doi: 10.1016/j.saa.2022.121286. Epub 2022 Apr 21.
Traumatic delayed splenic rupture often follows by a "latent period" without typical symptoms after injury. During this period, though there are no obvious symptoms, the injury is still present and changing. In this study, we constructed an SD rat model of delayed splenic rupture; evaluated the model by HE staining, Perl's staining, Masson trichrome staining and immunohistochemical staining; observed the pathological changes of spleen tissue in delayed splenic rupture at different times after splenic injury; we found that pathological change of injured tissues were different from non-injured, and has phases-change patterns, it can be roughly divided into three phases: 2-7 d, 10-14 d, and 18-28.We then investigated the relationship between the pathological changes and FTIR spectroscopy by chemometric methods. The main distinction of injured and non-injured tissue was the protein secondary structure of amide I, and the main distinctions of different phases of delayed splenic rupture were protein secondary structures and content of amide I and amide II.A classification model developed by SVM-DA was used to infer three phases (2-7 days, 10-12 days and 14-28 days). According to the most probable class, the accuracy of external validation is 96.7%. The results indicate that FTIR spectroscopy combined with various types of pathological staining has a potential for forensic identification and can provide theoretical support and diagnostic reference on clinical persistent injury.
创伤性延迟性脾破裂在受伤后常经历一段“潜伏期”，无典型症状。在此期间，尽管没有明显的症状，但损伤仍然存在并在不断变化。在这项研究中，我们构建了 SD 大鼠延迟性脾破裂模型；通过 HE 染色、Perl 染色、Masson 三色染色和免疫组织化学染色对模型进行评估；观察不同时间点脾损伤后延迟性脾破裂脾组织的病理变化；我们发现，损伤组织的病理变化与未损伤组织不同，具有阶段性变化模式，大致可分为三个阶段：2-7d、10-14d 和 18-28d。然后，我们通过化学计量学方法研究了病理变化与 FTIR 光谱之间的关系。损伤和未损伤组织的主要区别在于酰胺 I 的蛋白质二级结构，延迟性脾破裂不同阶段的主要区别在于酰胺 I 和酰胺 II 的蛋白质二级结构和含量。使用 SVM-DA 开发的分类模型来推断三个阶段（2-7 天、10-12 天和 14-28 天）。根据最可能的类别，外部验证的准确率为 96.7%。结果表明，FTIR 光谱结合各种类型的病理染色具有法医鉴定的潜力，可以为临床持续性损伤提供理论支持和诊断参考。
