氯己定释放弹性体对抗口腔微宇宙生物膜的抗生物膜活性。

Anti-biofilm activity of chlorhexidine-releasing elastomerics against dental microcosm biofilms.

机构信息

Department of Preventive Dentistry and Public Oral Health, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.

Department of Dental Hygiene, College of Medical Convergence, Catholic Kwandong University, 25601, 24, Beomil-ro 579 beon-gil, Gangneung-si, Gangwon-do, Republic of Korea.

出版信息

J Dent. 2022 Jul;122:104153. doi: 10.1016/j.jdent.2022.104153. Epub 2022 May 5.

DOI:10.1016/j.jdent.2022.104153

PMID:35526753

Abstract

OBJECTIVE

To evaluate the anti-biofilm activity of chlorhexidine-releasing elastomerics (CRE) developed to prevent biofilm-related diseases in orthodontic patients, using dental microcosm biofilms.

METHODS

Elastomerics coated with one of two solutions (CRE 1 and 2) were attached to bovine enamel specimens. Uncoated elastomerics were used for negative (distilled water [DW]) and positive (0.1% chlorhexidine [CHX]) control groups. After saliva inoculation on the surface of the specimen for biofilm formation, DW and CRE groups were treated with DW, and the positive control group was treated with CHX twice a day for 5 min. After 7 days of biofilm formation, colony-forming units (CFUs, total and aciduric bacteria), red/green (R/G) ratio, biofilm thickness, live/dead cell ratio, and bacterial morphology in the biofilms were evaluated. Enamel demineralization was evaluated by fluorescence loss (ΔF).

RESULTS

The CFUs of total and aciduric bacteria and R/G ratios in the CRE groups were significantly lower than those in the DW group with a reduction by 13%, 13%, and 19%, respectively (p < 0.05). The CFUs of total bacteria was significantly lower in the CRE groups than in the 0.1% CHX group (p < 0.05). Among the CRE groups, only CRE 1 exhibited a significantly reduced biofilm thickness of 54% compared to the DW group (p < 0.05) and apparent changes in bacterial morphology. ΔF in the CRE groups was significantly higher by 36% compared to that in the DW group (p < 0.05).

CONCLUSIONS

CREs exhibited anti-biofilm and demineralization-inhibiting effect. Particularly, CRE 1 using dichloromethane as the solvent was most effective against biofilms.

CLINICAL SIGNIFICANCE

Chlorhexidine-releasing elastomerics exhibited increased anti-biofilm and demineralization-inhibiting effect compared to 0.1% chlorhexidine mouthwash. Therefore, it is possible to prevent biofilm-related diseases simply and effectively by applying chlorhexidine-releasing elastomerics to orthodontic patients.

摘要

目的

使用牙菌斑微宇宙评估为预防正畸患者生物膜相关疾病而开发的含氯己定弹性体（CRE）的抗生物膜活性。

方法

将涂有两种溶液之一的弹性体（CRE1 和 2）附着在牛牙釉质标本上。未涂覆的弹性体用作阴性（蒸馏水[DW]）和阳性（0.1%氯己定[CHX]）对照组。在标本表面接种唾液以形成生物膜后，DW 和 CRE 组用 DW 处理，阳性对照组每天用 CHX 处理两次，每次 5 分钟。形成生物膜 7 天后，评估生物膜中的菌落形成单位（CFU，总细菌和耐酸菌）、红/绿（R/G）比值、生物膜厚度、活/死细胞比值和细菌形态。通过荧光损失（ΔF）评估牙釉质脱矿。

结果

与 DW 组相比，CRE 组的总细菌和耐酸菌的 CFU 和 R/G 比值分别降低了 13%、13%和 19%（p<0.05）。CRE 组的总细菌 CFU 明显低于 0.1%CHX 组（p<0.05）。在 CRE 组中，只有 CRE1 与 DW 组相比，生物膜厚度显著降低了 54%（p<0.05），并且细菌形态发生明显变化。与 DW 组相比，CRE 组的 ΔF 增加了 36%（p<0.05）。