Department of Health Science, Gachon University Graduate School of Public Health, Incheon 21936, Republic of Korea.
Department of Electronic Engineering, Gachon University, Seongnam-si 13120, Republic of Korea.
Int J Environ Res Public Health. 2023 Jan 30;20(3):2447. doi: 10.3390/ijerph20032447.
We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 days. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine (CHX) for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity of the biofilms was measured as red/green ratios (Ratio) before and after treatment. Total and aciduric bacteria were counted as colony-forming units. Using live/dead bacterial staining, bacterial viability was calculated as the Ratio. Ratio was approximately 0.91-fold lower in the NTAPP group than in the CHX group on day 1 of treatment ( = 0.001), and approximately 0.94-fold lower on both days 2 and 3 ( < 0.001). The number of total bacteria was higher in the NTAPP group than in the CHX group, but not significantly different. The number of aciduric bacteria was lowest in the CHX group ( < 0.001). However, bacterial viability was lowest in the NTAPP group. Restricted bacterial aggregation was observed in the NTAPP group. These findings suggest that NTAPP may more effectively reduce the pathogenicity of oral microcosm biofilms than 0.12% CHX.
我们比较评估了非热常压等离子体（NTAPP）对口腔微生态生物膜的抗菌效果。口腔微生态生物膜源自人唾液接种，在 48 羟磷灰石盘上培养 6 天。将制备好的生物膜分为三组，分别为蒸馏水处理 1 分钟、0.12%洗必泰（CHX）处理 1 分钟和 NTAPP 处理 5 分钟。使用定量光致荧光-数字相机，测量处理前后生物膜的红色荧光强度，以红/绿比值（Ratio）表示。总菌和耐酸菌以菌落形成单位计数。使用活/死细菌染色，计算细菌活力作为 Ratio。处理第 1 天，NTAPP 组的 Ratio 比 CHX 组低约 0.91 倍（= 0.001），第 2 天和第 3 天的 Ratio 低约 0.94 倍（<0.001）。NTAPP 组的总菌数量高于 CHX 组，但无统计学差异。耐酸菌数量在 CHX 组最低（<0.001）。然而，NTAPP 组的细菌活力最低。NTAPP 组观察到细菌聚集受限。这些发现表明，NTAPP 可能比 0.12% CHX 更有效地降低口腔微生态生物膜的致病性。
