Wang Y, Xing M
Institute of Genetics and Cytology, Northeast Normal University, Changchun.
Yi Chuan Xue Bao. 1999;26(2):150-6.
Opinions upon whether actin is present in synaptonemal complexes of eukaryotes are still controversial. We detected actin in synaptonemal complexes of spermatocytes of Mesocricetus auratus with indirect immunofluorescence technique and observed bright fluorescence in the SCs, indicating the presence of actin in the structure. We labelled sections of the spermatocytes of M. auratus with immunogold technique, gold particels were observed over the lateral elements and attachment plaques of the SCs, and many of the gold were found in clusters, confirming the results of the immunofluorescence observations. Further examinations revealed the gold particles representing actin in SCs of autosomes and sex chromosomes and also in SCs of zygotene and pachytene. Our results are in favour of the point of view that actin is a component of the SC.
关于肌动蛋白是否存在于真核生物的联会复合体中，目前仍存在争议。我们用间接免疫荧光技术在金黄地鼠精母细胞的联会复合体中检测到了肌动蛋白，并在联会复合体中观察到明亮的荧光，表明该结构中存在肌动蛋白。我们用免疫金技术标记了金黄地鼠精母细胞的切片，在联会复合体的侧生元件和附着斑上观察到了金颗粒，并且发现许多金颗粒聚集在一起，证实了免疫荧光观察的结果。进一步的检查发现，代表肌动蛋白的金颗粒存在于常染色体和性染色体的联会复合体中，也存在于偶线期和粗线期的联会复合体中。我们的结果支持肌动蛋白是联会复合体组成成分这一观点。
