俄罗斯库尔斯克地区由新型普马拉病毒进化分支引起的肾综合征出血热致死病例。

A fatal case of haemorrhagic fever with renal syndrome in Kursk Region, Russia, caused by a novel Puumala virus clade.

机构信息

Federal Budget Institution of Science "Central Research Institute of Epidemiology" of The Federal Service on Customers' Rights Protection and Human Well-being Surveillance, Moscow 111123, Russian Federation; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow 108819, Russian Federation.

The National Medical Research Center for Endocrinology, Moscow 117036, Russian Federation.

出版信息

Infect Genet Evol. 2022 Aug;102:105295. doi: 10.1016/j.meegid.2022.105295. Epub 2022 May 5.

DOI:10.1016/j.meegid.2022.105295

PMID:35526822

Abstract

Haemorrhagic fever with renal syndrome (HFRS) is the most widespread natural-focal human disease in the Russian Federation. In this study, we report virological assessment of a fatal case of HFRS-PUUV (Puumala virus) in the Kursk Region. The infection caused severe multiorgan failure and the maximum viral load was detected in the tissue of the spleen. Viral sequences were obtained from the patient's autopsy material and lung tissues of bank voles captured in the region. These sequences formed a new clade in the PUUV phylogenetic tree, an outgroup to all known Russian (RUS) lineage sequences. On the other hand viruses collected in the Kursk Region grouped with the RUS lineage and are separated from all other PUUV linages. We propose to nominate this novel group as W-RUS as the identified viruses were collected near the western Russian boundary. The recombination signals between their ancestors and RUS lineage representatives from the Volga region were revealed. The strain Samara_94/CG/2005 suggestively emerged as the result of reassortment between the ancestors of W-RUS and DTK-Ufa-97.

摘要

肾综合征出血热（HFRS）是俄罗斯联邦分布最广的自然疫源性人类疾病。本研究报告了库尔斯克地区一例致命性 HFRS-PUUV（普马拉病毒）病例的病毒学评估。该感染导致严重的多器官衰竭，脾脏组织中检测到的病毒载量最高。从患者尸检材料和该地区捕获的林姬鼠肺组织中获得了病毒序列。这些序列在 PUUV 系统发育树中形成了一个新的分支，与所有已知的俄罗斯（RUS）谱系序列不同。另一方面，在库尔斯克地区采集的病毒与 RUS 谱系聚集在一起，与所有其他 PUUV 谱系分离。我们建议将这个新的群组命名为 W-RUS，因为所鉴定的病毒是在俄罗斯西部边界附近采集的。在它们的祖先与来自伏尔加地区的 RUS 谱系代表之间发现了重组信号。菌株 Samara_94/CG/2005 提示性地出现在 W-RUS 和 DTK-Ufa-97 的祖先之间的重组的结果。