Department of International Agricultural Development, Graduate School of International Food and Agricultural Studies, Tokyo University of Agriculture.
Department of Molecular Microbiology, Faculty of Life Sciences, Tokyo University of Agriculture.
Microbes Environ. 2022;37(2). doi: 10.1264/jsme2.ME21062.
The bacterial community of water yam (Dioscorea alata L.) cv. A-19 is vital because it may promote plant growth without the need for fertilization. However, the influence of fertilization practices on the composition and proportion of the bacterial community of water yam cv. A-19 has not yet been extensively examined. Therefore, we herein investigated the diversity and composition of the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization using amplicon community profiling based on 16S rRNA gene sequences. No significant difference was detected in the growth of plants cultivated with or without chemical fertilization. Alpha diversity indices were significantly dependent on each compartment, and a decrease was observed in indices from the belowground (rhizosphere and root) to aboveground compartments (stem and leaf). The bacterial composition of each compartment was clustered into three groups: bulk soil, rhizosphere and root, and stem and leaf. Chemical fertilization did not significantly influence the diversity or composition of the water yam cv. A-19 bacterial community. It remained robust in plants cultivated with chemical fertilization. The amplicon community profiling of bacterial communities also revealed the dominance of two bacterial clades, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Burkholderia-Caballeronia-Paraburkholderia clade, with and without chemical fertilization. This is the first study to characterize the bacterial community of water yam cv. A-19 cultivated with and without chemical fertilization.
水芋（Dioscorea alata L.） cv. A-19 的细菌群落至关重要，因为它可以在无需施肥的情况下促进植物生长。然而，施肥实践对水芋 cv. A-19 细菌群落的组成和比例的影响尚未得到广泛研究。因此，我们使用基于 16S rRNA 基因序列的扩增子群落分析，研究了有和没有化学施肥的水芋 cv. A-19 细菌群落的多样性和组成。有和没有化学施肥的植物生长没有显著差异。α多样性指数显著依赖于每个隔室，并且从地下（根际和根）到地上隔室（茎和叶）观察到指数下降。每个隔室的细菌组成聚类为三组：土壤、根际和根、茎和叶。化学施肥对水芋 cv. A-19 细菌群落的多样性或组成没有显著影响。它在有化学施肥的植物中仍然保持稳健。细菌群落的扩增子社区分析还揭示了两个细菌类群的优势，即 Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium 类群和 Burkholderia-Caballeronia-Paraburkholderia 类群，有和没有化学施肥。这是首次对有和没有化学施肥的水芋 cv. A-19 细菌群落进行特征描述的研究。
