Weitzel G, Pilatus U, Rensing L
Exp Cell Res. 1987 May;170(1):64-79. doi: 10.1016/0014-4827(87)90117-0.
In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature increases (23-32 degrees C), addition of 2,4-dinitrophenol (greater than 1 mM), sodium arsenite (greater than 3.75 X 10(-5) M) or sodium cyanide (greater than 10 mM]. Changes in the intracellular pH occur within 5 min after treatment, attain a maximum within 30 min and are subsequently stable. HSPs 98, 85 and 70 show maximum synthesis rates 1-2 h after a 40 degrees C heat shock. The synthesis rates then decline. HSPs 56, 44 and 33 reveal a smaller and slower increase and almost no decrease in the synthesis rate within 4 h at 40 degrees C. The similar dose dependencies of HSP synthesis and cytoplasmic pH. as well as the immediate response of the pH, can also be demonstrated in the mitochondrial mutant of S. cerevisiae (Q0). This result indicates that the heat-shock response is mainly independent of intact oxidative phosphorylation. No correlation was observed between HSP synthesis rate and total intracellular ATP content.
在酿酒酵母中，通过[31P]核磁共振光谱法测定发现，热休克蛋白（HSP）合成的诱导伴随着细胞质和液泡pH值的降低。在多种处理条件下（温度升高（23 - 32摄氏度）、添加2,4 - 二硝基苯酚（大于1 mM）、亚砷酸钠（大于3.75×10(-5) M）或氰化钠（大于10 mM）），HSP合成与细胞质pH值酸化之间的关系呈剂量依赖性。处理后5分钟内细胞内pH值发生变化，30分钟内达到最大值，随后保持稳定。在40摄氏度热休克后1 - 2小时，HSP 98、85和70显示出最大合成速率。随后合成速率下降。在40摄氏度下4小时内，HSP 56、44和33显示出较小且较慢的增加，合成速率几乎没有下降。HSP合成与细胞质pH值的类似剂量依赖性以及pH值的即时响应，也可以在酿酒酵母的线粒体突变体（Q0）中得到证明。这一结果表明热休克反应主要独立于完整的氧化磷酸化。未观察到HSP合成速率与细胞内总ATP含量之间的相关性。
