School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
School of Materials Science and Engineering, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
Biomater Adv. 2022 Feb;133:112604. doi: 10.1016/j.msec.2021.112604. Epub 2021 Dec 9.
Acute skin injury should be treated in time, due to many factors will affect the normal healing process of wounds, hinder tissue regeneration, and eventually form chronic wounds. Herein, an injectable, in-situ gel-forming hydrogel has been fabricated. The hydrogel is stabilized by dynamic Schiff base and composed of gelatin (Gel), oxidized dextran (Odex) and apocynin (Apo). In vitro studies have shown that this hydrogel has good injectability, plasticity, self-healing and efficient hemostatic properties. The hydrogel has good cytocompatibility with HaCaT and L929 cells by the Live/Dead cell staining. Furthermore, in vivo studies have shown that hydrogel loaded with APO can accelerate angiogenesis and skin tissue regeneration by reducing wound inflammation. Therefore, the injectable Gel/Odex/Apo hydrogel has the advantages of simple preparation process, convenient use and multifunction, etc., it is a promising wound dressing for full-thickness skin repair and has great potential in the field of skin tissue regeneration.
急性皮肤损伤应及时治疗，由于多种因素会影响伤口的正常愈合过程，阻碍组织再生，最终形成慢性伤口。在此，制备了一种可注射的原位凝胶形成水凝胶。该水凝胶由动态席夫碱稳定，由明胶（Gel）、氧化葡聚糖（Odex）和白藜芦醇（Apo）组成。体外研究表明，该水凝胶具有良好的可注射性、可塑性、自修复性和高效止血性能。水凝胶通过 Live/Dead 细胞染色与 HaCaT 和 L929 细胞具有良好的细胞相容性。此外，体内研究表明，载有 APO 的水凝胶通过减少伤口炎症，可加速血管生成和皮肤组织再生。因此，可注射的 Gel/Odex/Apo 水凝胶具有制备工艺简单、使用方便、多功能等优点，是一种有前途的全厚皮肤修复用创面敷料，在皮肤组织再生领域具有巨大潜力。
