Aussel C, Desmoulins D, Agneray J, Ekindjian O G
FEBS Lett. 1987 Apr 20;214(2):327-30. doi: 10.1016/0014-5793(87)80080-7.
The biological sensitivity of cultured non-rheumatoid human synovial cells (NRSCs) and rheumatoid synovial cells (RSCs) was examined in terms of the ability of insulin to stimulate the uptake of alpha-aminoisobutyrate (AIB). NRSCs, like numerous fibroblastic lines, were sensitive to physiological concentrations of the hormone: half-maximal stimulation was obtained with (4 X 10(-10) M) insulin, while maximum transport was found with a 60-90 min association time. On the contrary, although the basal transport was similar in RSCs, insulin was totally unable to accelerate AIB transport in these cells. Inflammatory processes lead to an insulin resistance which most likely involves a post-receptor step at the cellular level.
通过检测胰岛素刺激α-氨基异丁酸（AIB）摄取的能力，研究了培养的非类风湿性人滑膜细胞（NRSCs）和类风湿性滑膜细胞（RSCs）的生物学敏感性。与许多成纤维细胞系一样，NRSCs对生理浓度的激素敏感：胰岛素浓度为（4×10⁻¹⁰ M）时可获得半数最大刺激，而在60 - 90分钟的结合时间可达到最大转运。相反，尽管RSCs中的基础转运相似，但胰岛素完全无法加速这些细胞中的AIB转运。炎症过程导致胰岛素抵抗，这很可能涉及细胞水平上受体后的步骤。
