Chemistry and Materials School, Jinan University, Guangzhou, China.
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China.
J Food Biochem. 2022 Aug;46(8):e14196. doi: 10.1111/jfbc.14196. Epub 2022 May 8.
Oxidative stress-induced dysfunction of nerve cells has been implicated as a crucial cause of cell death in neurodegenerative diseases. In Asian countries, herbs, such as Angelica sinensis (Oliv.) Diels (DG) and Rehmannia glutinosa (Gaertn.) DC. (SDH), have long be considered to have antiaging abilities. The herbs act as neuro protectants that rescue nerve cells from oxidative stress damage and apoptosis. Thus, developing herbal formulas can potentially lead to new treatments for neurodegenerative diseases. In this study, we compared the effective active components and antioxidant properties of extractive of DG and SDH (DG-SDH) when formulated at different ratios. DG-SDH formulated at a ratio of 3:2 (DG-SDH [3:2]) produced the highest content of polysaccharides, polyphenols, and flavonoids. It also showed the best ability in removing DPPH and hydroxyl free radicals compared to single herb or other compounding ratio. The antioxidant activity of DG-SDH (3:2) showed best synergistic effects in scavenging activity assays of DPPH free radicals and hydroxyl free radicals. DG-SDH (3:2) could increase the cell viability of SHSY-5Y cells, PC-12 cells, and BV-2 cells. In particular, DG-SDH (3:2) protected SHSY-5Y cells from H O -induced cell injury by inhibiting excessive expression of reactive oxygen species (ROS), reducing the rate of apoptosis and restoring mitochondrial membrane potential. Actin-Tracker Green and DAPI staining and fluorescence microscope observation confirmed that DG-SDH (3:2) helped in preserving cell morphology under oxidative stress. These findings support that DG-SDH (3:2) promote the neuroprotection against hydrogen peroxide and can serve as a novel therapy for neurodegenerative diseases. PRACTICAL APPLICATIONS: This is the first study to investigate DG and SDH interaction between effective ingredients. These findings support that DG-SDH (3:2) has the best synergistic effects in antioxidant activity and promote the neuroprotection against hydrogen peroxide. Hence, DG-SDH (3:2) will be an excellent candidate to be developed as a functional food ingredients or nutraceuticals for neurodegenerative diseases.
氧化应激诱导的神经细胞功能障碍被认为是神经退行性疾病中细胞死亡的一个关键原因。在亚洲国家，当归（Angelica sinensis（Oliv.）Diels（DG））和地黄（Rehmannia glutinosa（Gaertn.）DC.（SDH））等草药长期以来被认为具有抗衰老作用。这些草药作为神经保护剂，可以挽救神经细胞免受氧化应激损伤和细胞凋亡。因此，开发草药配方可能会为神经退行性疾病的治疗提供新的方法。在这项研究中，我们比较了以不同比例配方的当归和地黄提取物（DG-SDH）的有效活性成分和抗氧化特性。当以 3:2 的比例配方时，DG-SDH 产生的多糖、多酚和类黄酮含量最高。与单一草药或其他化合物比例相比，它在清除 DPPH 和羟基自由基方面也表现出最好的能力。DG-SDH（3:2）在 DPPH 自由基和羟基自由基清除活性测定中表现出最佳的协同作用。DG-SDH（3:2）可以提高 SHSY-5Y 细胞、PC-12 细胞和 BV-2 细胞的细胞活力。特别是，DG-SDH（3:2）通过抑制活性氧（ROS）的过度表达、降低细胞凋亡率和恢复线粒体膜电位来保护 SHSY-5Y 细胞免受 H2O2 诱导的细胞损伤。肌动蛋白追踪绿色和 DAPI 染色以及荧光显微镜观察证实，DG-SDH（3:2）有助于在氧化应激下保持细胞形态。这些发现支持 DG-SDH（3:2）可以促进对过氧化氢的神经保护作用，并可以作为神经退行性疾病的一种新的治疗方法。实际应用：这是第一项研究 DG 和 SDH 之间有效成分相互作用的研究。这些发现支持 DG-SDH（3:2）在抗氧化活性方面具有最佳的协同作用，并促进对过氧化氢的神经保护作用。因此，DG-SDH（3:2）将是开发神经退行性疾病功能性食品成分或营养保健品的优秀候选者。
