当归中分离得到的多糖组分及其抗氧化特性
Component and antioxidant properties of polysaccharide fractions isolated from Angelica sinensis (OLIV.) DIELS.
作者信息
Yang Xingbin, Zhao Yan, Zhou Yanjuan, Lv You, Mao Jieli, Zhao Ping
机构信息
Key Laboratory of Ministry of Education for Medicinal Plant Resource and Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
出版信息
Biol Pharm Bull. 2007 Oct;30(10):1884-90. doi: 10.1248/bpb.30.1884.
An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose and galacturonic acid as well as trace amount of mannose and glucuronic acid, and the fractionation altered significantly the distribution of component monosaccharides in APFs. APF3 was the most active fraction to effectively inhibit H(2)O(2)-caused decrease of cell viability, lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation, and also reduced H(2)O(2)-caused decline of superoxide dismutase (SOD) activity and glutathione (GSH) depletion (p<0.05), followed by APF2 and APF1 in decreasing order. Furthermore, it was found that APFs (100 microg/ml) could protect macrophages by inhibiting the release of excess NO and reactive oxygen species (ROS) induced by high concentrations of H(2)O(2) (0.8-1.6 mM).
建立了一种高效毛细管电泳（HPCE）分析方法，用于同时分离和鉴定当归多糖级分（APF）中的单糖成分，即APF1、APF2和APF3。APF中的主要糖类被鉴定为阿拉伯糖、葡萄糖、鼠李糖、半乳糖和半乳糖醛酸，以及痕量的甘露糖和葡萄糖醛酸，并且分级分离显著改变了APF中各单糖成分的分布。APF3是最具活性的级分，能有效抑制H₂O₂引起的细胞活力下降、乳酸脱氢酶（LDH）泄漏和丙二醛（MDA）形成，还能减轻H₂O₂引起的超氧化物歧化酶（SOD）活性下降和谷胱甘肽（GSH）消耗（p<0.05），其次是APF2和APF1，活性依次降低。此外，还发现APF（100μg/ml）可通过抑制高浓度H₂O₂（0.8 - 1.6 mM）诱导的过量一氧化氮（NO）和活性氧（ROS）释放来保护巨噬细胞。
Zotero 插件