Center for Fisheries, Aquaculture, and Aquatic Sciences, Southern Illinois University-Carbondale, Carbondale, Illinois, USA.
School of Biological Sciences, Southern Illinois University-Carbondale, Carbondale, Illinois, USA.
Zebrafish. 2022 Jun;19(3):94-103. doi: 10.1089/zeb.2022.0002. Epub 2022 May 6.
Manipulating feeding rate and protein quality may improve growth and feeding efficiency of cultured species. However, whether feeding rate, protein quality, or their interaction has a greater effect on growth and feeding efficiency response variables is unknown. To determine whether feeding rate and protein quality individually or interactively affect growth and feeding efficiency, juvenile Zebrafish () were either offered nutritionally similar diet consisting of either menhaden fishmeal protein or a 100% replacement of fishmeal with soybean meal-based protein restrictively or to satiation. Total length, weight, feed intake, and feed conversion ratio (FCR) were measured throughout the duration of the study. Protein quality and feeding rate individually and interactively affected feed intake and FCR: Zebrafish offered feed to satiation had higher growth and FCR than those fed restrictively, and Zebrafish fed soybean meal-based diet showed lower growth and higher FCR and feed intake compared to those fed fishmeal-based diet, although magnitude of response depended on feeding rate. These findings likely indicate lower digestibility of soybean meal or the presence of antinutritional factors in soybean meal that led to impaired nutrient absorption of fish offered soybean meal-based diet. Differences in measured response variables between protein qualities and feeding rates highlight the importance of determining interactive effects in nutritional studies.
调控摄食率和蛋白质质量可能会改善养殖物种的生长和摄食效率。然而，摄食率、蛋白质质量或它们的相互作用对生长和摄食效率的影响更大尚不清楚。为了确定摄食率和蛋白质质量是否单独或相互作用影响生长和摄食效率，我们用限制或饱食的方式给幼期斑马鱼投喂营养相似的饲料，这些饲料分别由鲱鱼鱼粉蛋白或 100%的大豆蛋白粉替代鱼粉制成。在整个研究过程中，我们测量了斑马鱼的全长、体重、摄食量和饲料转化率（FCR）。蛋白质质量和摄食率单独和相互作用影响摄食量和 FCR：饱食组的斑马鱼生长和 FCR高于限制组，而与投喂鱼粉基饲料的斑马鱼相比，投喂大豆蛋白粉基饲料的斑马鱼生长较慢、FCR 和摄食量较高，尽管反应的幅度取决于摄食率。这些发现可能表明大豆蛋白粉的消化率较低，或者大豆蛋白粉中存在抗营养因子，导致投喂大豆蛋白粉基饲料的鱼类营养物质吸收受损。蛋白质质量和摄食率之间的测量响应变量的差异突出了在营养研究中确定相互作用效应的重要性。
