Center for Fisheries, Aquaculture, and Aquatic Sciences, School of Biological Sciences, Southern Illinois University, Carbondale, Illinois, USA.
A. Watson Armour III Center for Animal Health and Welfare, John G. Shedd Aquarium, Chicago, Illinois, USA.
Zebrafish. 2021 Apr;18(2):125-138. doi: 10.1089/zeb.2020.1952. Epub 2021 Mar 23.
Nutritional programming (NP) is considered a promising approach that can counteract the negative effects of dietary plant protein (PP) by introducing PP to fish in the early developmental stages. Therefore the objective of our study was to assess the effect of NP on PP utilization and the gut microbiome in zebrafish . The study included four treatment groups: (1) a positive control group that received a fishmeal (FM) diet throughout the entire trial (+ control); (2) a negative control group that received PP diet throughout the entire trial (- control); (3) an NP group that received dietary PP during the larval stage followed by FM-based diet during the juvenile stage and PP diet again during a PP challenge in the grow-out phase (NP-PP); and (4) an FM-group that received FM-based diet during the larval and juvenile stages and was challenged with a PP diet during the grow-out phase (NP-FM). During the PP challenge, the NP-PP group achieved the highest weight gain compared to the (-) control and NP-FM groups. The relative abundance of certain phyla such as Chloroflexi, Planctomycetes, and Bacteroidetes presented higher values in some groups at early juvenile stage. The fish gut microbiome also presented differences throughout the study.
营养编程（NP）被认为是一种很有前途的方法，可以通过在早期发育阶段将植物蛋白（PP）引入鱼类来抵消膳食植物蛋白的负面影响。因此，我们的研究目的是评估 NP 对斑马鱼中 PP 利用和肠道微生物组的影响。研究包括四个处理组：（1）在整个试验中都接受鱼粉（FM）饮食的阳性对照组（+ 对照组）；（2）在整个试验中都接受 PP 饮食的阴性对照组（- 对照组）；（3）在幼虫阶段接受 PP 饮食，然后在幼鱼阶段和 PP 挑战阶段接受基于 FM 的饮食，在生长阶段再次接受 PP 饮食的 NP 组（NP-PP）；（4）在幼虫和幼鱼阶段接受基于 FM 的饮食，在生长阶段接受 PP 饮食挑战的 FM 组（NP-FM）。在 PP 挑战期间，NP-PP 组与（-）对照组和 NP-FM 组相比，体重增加最高。某些门如绿弯菌门、浮霉菌门和拟杆菌门的相对丰度在早期幼鱼阶段的某些组中更高。整个研究过程中，鱼类肠道微生物组也存在差异。
