Di Iorio Daniele, Marti Almudena, Koeman Sander, Huskens Jurriaan
Molecular NanoFabrication Group, MESA+ Institute for Nanotechnology, University of Twente Enschede The Netherlands
RSC Adv. 2019 Nov 4;9(61):35608-35613. doi: 10.1039/c9ra08714a. eCollection 2019 Oct 31.
Biomolecules are immobilized onto surfaces employing the fast and stable adsorption of poly-l-lysine (PLL) polymers and the versatile copper-free click chemistry reactions. This method provides the combined advantages of versatile surface adsorption with density control using polyelectrolytes and of the covalent and orthogonal immobilization of biomolecules with higher reaction rates and improved yields of click chemistry. Using DNA attachment as a proof of concept, control over the DNA probe density and applicability in electrochemical detection are presented.
生物分子通过聚-L-赖氨酸（PLL）聚合物的快速稳定吸附以及通用的无铜点击化学反应固定在表面上。该方法结合了使用聚电解质进行通用表面吸附和密度控制的优点，以及生物分子的共价和正交固定化的优点，具有更高的反应速率和更好的点击化学产率。以DNA附着作为概念验证，展示了对DNA探针密度的控制及其在电化学检测中的适用性。
