Mun Seong Hee, Oh Hyeon Ji, Kwon Joon Yeong
Dept. of Aquatic Life Medical Science, Sunmoon University, Asan 31460, Korea.
Dev Reprod. 2022 Mar;26(1):13-21. doi: 10.12717/DR.2022.26.1.13. Epub 2022 Mar 31.
Neurokinin B (NKB) is a neuropeptide involved in the regulation of reproductive endocrine system of vertebrate animals, including fish. However, the pathway of NKB action in fish has not been clearly elucidated. In order to clarify the effect of NKB and NKF (neurokinin F) on gonadotropic hormone (GTH) gene expression in the pituitary, we studied the changes of LHβ and FSHβ gene expressions by using two different pituitary culture methods (whole pituitary culture or dispersed pituitary cell culture). Pituitaries were removed from mature female and male Nile tilapia. Changes of LHβ and FSHβ gene expressions were measured and compared after the treatment with NKB or NKF peptides at concentrations 0 to 1,000 nM. Expression of GTH genes in the whole pituitary cultures treated with NKB or NKF peptides did not show significant difference except in female at one concentration when treated with NKF. On the contrary, there were significant changes of GTH gene expressions in the dispersed pituitary cell cultures when treated with NKB and NKF peptides. These results suggest that dispersed pituitary cell culture is more relevant than whole pituitary culture in studying the function of pituitary, and that NKB and NKF could act directly on the pituitary to regulate the expression of GTH genes.
神经激肽B（NKB）是一种神经肽，参与包括鱼类在内的脊椎动物生殖内分泌系统的调节。然而，鱼类中NKB的作用途径尚未明确阐明。为了阐明NKB和神经激肽F（NKF）对垂体中促性腺激素（GTH）基因表达的影响，我们采用两种不同的垂体培养方法（全垂体培养或分散垂体细胞培养）研究了促黄体生成素β（LHβ）和促卵泡生成素β（FSHβ）基因表达的变化。从成熟的尼罗罗非鱼雌雄个体中取出垂体。用浓度为0至1000 nM的NKB或NKF肽处理后，测量并比较LHβ和FSHβ基因表达的变化。用NKB或NKF肽处理的全垂体培养物中，GTH基因的表达除了用NKF处理的一种浓度下的雌性个体外，没有显示出显著差异。相反，用NKB和NKF肽处理分散垂体细胞培养物时，GTH基因表达有显著变化。这些结果表明，在研究垂体功能方面，分散垂体细胞培养比全垂体培养更具相关性，并且NKB和NKF可以直接作用于垂体来调节GTH基因的表达。
