Xiong Sijia, Gao Huichang, Qin Lanfeng, Jia Yongguang, Gao Meng, Ren Li
School of Materials Science and Engineering, South China University of Technology Guangzhou 510641 China
National Engineering Research Centre for Tissue Restoration and Reconstruction Guangzhou 510006 China.
RSC Adv. 2019 Sep 18;9(50):29463-29473. doi: 10.1039/c9ra04009a. eCollection 2019 Sep 13.
Collagen is a promising material for corneal tissue engineering. The surface topography of collagen is critical for cornea reconstruction and regeneration. Herein, we fabricated collagen films with microgrooved surface to investigate the effect of collagen film topography on corneal cell migration and antifibrosis. We found that the patterned films with microscale grooves could greatly affect the orientation, proliferation, migration, and gene expression of rabbit corneal epithelial cells and keratocytes. Compared with the natural cornea, the optimized collagen films with microgroove pattern show similar swelling performance, optical clarity, and biodegradability, which could efficiently promote the epithelial cell migration, accelerate wound healing process, and inhibit the fibrosis of keratocytes. These results suggest that collagen films with microgroove pattern are promising in corneal tissue engineering.
胶原蛋白是用于角膜组织工程的一种很有前景的材料。胶原蛋白的表面形貌对于角膜重建和再生至关重要。在此，我们制备了具有微槽表面的胶原蛋白膜，以研究胶原蛋白膜形貌对角膜细胞迁移和抗纤维化的影响。我们发现具有微米级凹槽的图案化膜可极大地影响兔角膜上皮细胞和角膜细胞的取向、增殖、迁移及基因表达。与天然角膜相比，具有微槽图案的优化胶原蛋白膜表现出相似的溶胀性能、光学清晰度和生物降解性，其可有效促进上皮细胞迁移、加速伤口愈合过程并抑制角膜细胞纤维化。这些结果表明，具有微槽图案的胶原蛋白膜在角膜组织工程中具有前景。
