Behera Nibedita, Arakha Manoranjan, Priyadarshinee Mamali, Pattanayak Biraja S, Soren Siba, Jha Suman, Mallick Bairagi C
Department of Chemistry, Ravenshaw University Cuttack-753003 Odisha India
Centre for Biotechnology, Siksha 'O' Anusandhan (Deemed to be University) Bhubaneswar-751003 Odisha India.
RSC Adv. 2019 Aug 12;9(43):24888-24894. doi: 10.1039/c9ra02082a. eCollection 2019 Aug 8.
Metal oxide nanoparticles (NPs) have shown enhanced antibacterial effects against many bacteria. Thus, understanding the potential antibacterial effects of nickel oxide nanoparticles (NiO NPs) against Gram-positive and Gram-negative pathogenic bacteria is an urgent need to enable the exploration of NiO NP use in biomedical sciences. To this end, NiO NPs were synthesized by microwave assisted hydrothermal synthesis method. The synthesized NPs were characterized by X-ray diffraction (XRD) and Fourier Transfer Infrared (FT-IR) and UV-visible spectroscopy. The morphological features of the synthesized NiO NPs were analysed using Transmission Electron Microscopy (TEM) and FE-SEM analysis. The antibacterial activity of NiO NP was explored using different antimicrobial and biophysical studies. The obtained data reveals that the NiO NP has stronger antibacterial activity against Gram-positive bacteria compared to Gram-negative bacteria. The mechanism behind the antibacterial activity of the NiO NP was explored by evaluating the amount of ROS generation at the NiO NP interface. The effect of ROS generation on the bacterial membrane was evaluated by BacLight assay and morphological analysis of the bacterial membrane using FE-SEM. The data altogether suggested that the oxidative stress generated at the NiO NP interface resulted in membrane damage leading to bacterial cell death.
金属氧化物纳米颗粒（NPs）已显示出对多种细菌增强的抗菌作用。因此，了解氧化镍纳米颗粒（NiO NPs）对革兰氏阳性和革兰氏阴性病原菌的潜在抗菌作用，对于探索NiO NPs在生物医学科学中的应用至关重要。为此，采用微波辅助水热合成法合成了NiO NPs。通过X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和紫外可见光谱对合成的NPs进行了表征。利用透射电子显微镜（TEM）和场发射扫描电子显微镜（FE-SEM）分析了合成的NiO NPs的形态特征。通过不同的抗菌和生物物理研究探索了NiO NP的抗菌活性。所得数据表明，与革兰氏阴性菌相比，NiO NP对革兰氏阳性菌具有更强的抗菌活性。通过评估NiO NP界面处活性氧（ROS）的生成量，探索了NiO NP抗菌活性的背后机制。通过BacLight检测和使用FE-SEM对细菌膜进行形态分析，评估了ROS生成对细菌膜的影响。总体数据表明，NiO NP界面处产生的氧化应激导致膜损伤，进而导致细菌细胞死亡。
