Emerging Materials for Energy and Environmental Applications Research Group, School of Engineering and Technology, Van Lang University, Ho Chi Minh City, Viet Nam.
Bio & NanoMaterials Lab | Drug Delivery and Controlled Release, Departamento de microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca, Chile; Center for Nanomedicine, Diagnostic & Drug Development (ND3), Universidad de Talca, Talca, Chile.
Food Chem Toxicol. 2022 Oct;168:113330. doi: 10.1016/j.fct.2022.113330. Epub 2022 Aug 1.
In currently, biosynthesis of copper oxide nanoparticles (CuO NPs) are most widely used numerous in biological applications such as biosensor, energy, medicine, agriculture, environmental and industrial wastewater treatment. The hierarchical CuO NPs was synthesized via green chemistry method by using of Abutilon indicum (A. indicum) leaf extract, its nontoxic, facile and low-cost approaches. Biogenic synthesized CuO NPs was characterized by using a UV-visible absorption spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Field mission scanning electron microscopy (FE-SEM) with Energy-dispersive X-ray spectroscopy (EDX) analysis. The synthesized CuO NPs was performed antibacterial activity against human pathogenic organisms of both Gram negative (Escherichia coli and Salmonella typhi) and Gram positive (Bacillus subtilis and Staphylococcus aureus) bacteria by using agar well diffusion method. Biological synthesized CuO NPs was showed potential bactericidal activity against Gram positive bacteria of B. subtilis than compared to Gram negative bacteria of E. coli. The cytotoxic effect of A. indicum mediated synthesized CuO NPs was evaluated against to human lung A549 and breast MDA-MB-231cancer cell lines by determined using of MTT assay. In furthermore, photocatalytic dye degradation was performed that synthesized CuO NPs have effectively removed 78% of malachite green dye molecule. Our investigation results suggested that the green synthesized CuO NPs potential biological activity of antibacterial activity against Gram positive bacterial, anticancer activity was effectively against MDA-MB-231cancer cell line and good dye degradation was exhibited in malachite green. The A. indicum aqueous leaf extract mediated synthesized CuO NPs has strongly suggested promising nano-biomaterials for fabrication of biomedical applications.
目前，氧化铜纳米粒子（CuO NPs）的生物合成在生物应用中最为广泛，如生物传感器、能源、医学、农业、环境和工业废水处理等领域。通过使用印度麻（A. indicum）叶提取物的绿色化学方法合成了分级 CuO NPs，这种方法具有无毒、简便和低成本的特点。生物合成的 CuO NPs 通过使用紫外可见吸收光谱（UV-Vis）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和场发射扫描电子显微镜（FE-SEM）与能谱分析（EDX）进行表征。通过琼脂孔扩散法对人类致病生物进行抗菌活性测试，评估了合成的 CuO NPs 对革兰氏阴性（大肠杆菌和伤寒沙门氏菌）和革兰氏阳性（枯草芽孢杆菌和金黄色葡萄球菌）细菌的抗菌活性。生物合成的 CuO NPs 对革兰氏阳性细菌枯草芽孢杆菌的杀菌活性比革兰氏阴性细菌大肠杆菌更强。通过 MTT 测定法评估了印度麻介导合成的 CuO NPs 对人肺 A549 和乳腺癌 MDA-MB-231 细胞系的细胞毒性作用。此外，还进行了光催化染料降解实验，结果表明，合成的 CuO NPs 能有效地去除 78%的孔雀石绿染料分子。我们的研究结果表明，绿色合成的 CuO NPs 具有潜在的抗菌活性，对革兰氏阳性细菌有效，对 MDA-MB-231 癌细胞系具有有效的抗癌活性，对孔雀石绿的染料降解效果良好。印度麻水提叶介导合成的 CuO NPs 强烈表明其作为生物医学应用的制造有前途的纳米生物材料。
