Zhang Yanhua, Huang Hui, Fu Hao, Zhao Meng, Wu Zhihua, Dong Yang, Li He, Duan Yourong, Sun Ying
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200032 China
Traditional Chinese Medicine Department, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200127 China.
RSC Adv. 2019 Oct 17;9(57):33302-33309. doi: 10.1039/c9ra03681d. eCollection 2019 Oct 15.
As known to all, ovarian cancer ranks the most lethal of the gynecological malignancies. The antitumor drugs based on platinum are first-line chemotherapy drugs for ovarian cancer. However, their therapeutic efficiency is severely limited owing to dose-limiting toxicities of platinum. New theranostic strategies to overcome chemotherapy toxicity is highly desirable. Meanwhile, the real-time treating effect is not visible for doctors. Herein, we constructed PFH/siRNA/FeO@Pt(iv) NPs-cRGD (NPs-cRGD) for precise theranostics against ovarian tumors with real-time imaging. The NPs-cRGD had a good storage stability and resisted the serum-induced aggregation, which was beneficial for drug delivery. Additionally, gel-retardation assay demonstrated that the NPs-cRGD exhibited great protection to siRNA to resist nuclease degradation. , the NPs-cRGD showed good dual-mode US/MRI imaging and the relative imaging research was also discussed. Moreover, the experiments indicated that the NPs-cRGD with US exhibited excellent antitumor therapeutic efficiency, resulting from the cRGD ligands and US exposure enhanced the cellular uptake efficiency. Thus, the dual-mode nanoparticles in this work may provide precious insight into the development of various multi-mode nanoplatforms delivering drugs or genes for precise theranostics against various cancer.
众所周知，卵巢癌是妇科恶性肿瘤中致死率最高的。基于铂的抗肿瘤药物是卵巢癌的一线化疗药物。然而，由于铂的剂量限制性毒性，它们的治疗效果受到严重限制。非常需要新的治疗诊断策略来克服化疗毒性。同时，医生无法看到实时治疗效果。在此，我们构建了PFH/siRNA/FeO@Pt(iv) NPs-cRGD（NPs-cRGD）用于对卵巢肿瘤进行精确的治疗诊断并实现实时成像。NPs-cRGD具有良好的储存稳定性，能抵抗血清诱导的聚集，这有利于药物递送。此外，凝胶阻滞试验表明NPs-cRGD对siRNA具有很好的保护作用，能抵抗核酸酶降解。 ，NPs-cRGD显示出良好的双模态超声/磁共振成像，并且还讨论了相关的成像研究。此外，实验表明，具有超声作用的NPs-cRGD表现出优异的抗肿瘤治疗效果，这是由于cRGD配体和超声暴露提高了细胞摄取效率。因此，这项工作中的双模态纳米颗粒可能为开发各种多模态纳米平台提供宝贵的见解，这些平台可用于递送药物或基因，以针对各种癌症进行精确的治疗诊断。
