Department of Pediatrics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Comput Math Methods Med. 2022 Apr 29;2022:7563281. doi: 10.1155/2022/7563281. eCollection 2022.
The febrile seizure (FS) is a common disease in emergency pediatrics, and about 30% of patients are children aged between 6 months and 5 years. Therefore, we aim to observe the protective impact of liraglutide (LIR) on brain injury in mice with FS and to explore its relevant mechanisms. Male SD mice were selected, and the FS model was established by heat bath method. The behavioral score was performed on mice with Racine grading, and nerve cells in apoptosis in the hippocampus were determined by TUNEL. The content of glutamate was determined by ELISA. mRNA levels and protein expression of GLP-1, GLP-1R, IL-1, IL-6, TNF-, and cleaved-caspase 3 were examined in mice by q-PCR and WB. Protein expression of -aminobutyric acid was influenced by WB as well. LIR prolonged the seizure latency and seizure duration in mice with FS. The GLP-1 and GLP-1R in the mouse hippocampus with FS expressed highly and also inhibited the number of nerve cells in apoptosis, decreased glutamate content, and increased -aminobutyric acid expression in the mouse hippocampus with FS. In addition, The IL-1, IL-6, and TNF-, in the mouse hippocampus with FS expressed to reduce with LIR. LIR is protective against brain injury in mice with FS and protects brain injury by inhibiting inflammatory factors in mice with FS. Our finding provides a reference for mitigating and delaying the development of FS as well as the prevention and treatment of brain injury caused by FS.
热性惊厥 (FS) 是儿科急诊中的一种常见疾病，约 30%的患者为 6 个月至 5 岁的儿童。因此，我们旨在观察利拉鲁肽 (LIR) 对 FS 小鼠脑损伤的保护作用，并探讨其相关机制。选择雄性 SD 小鼠，采用热浴法建立 FS 模型。对 Racine 分级的小鼠进行行为评分，并通过 TUNEL 检测海马区凋亡的神经细胞。通过 ELISA 测定谷氨酸含量。通过 q-PCR 和 WB 检测小鼠中 GLP-1、GLP-1R、IL-1、IL-6、TNF-和 cleaved-caspase 3 的 mRNA 水平和蛋白表达。WB 还影响 GABA 的蛋白表达。LIR 延长 FS 小鼠的惊厥潜伏期和惊厥持续时间。FS 小鼠海马中 GLP-1 和 GLP-1R 表达较高，还抑制神经细胞凋亡数量，降低 FS 小鼠海马中谷氨酸含量，增加 GABA 表达。此外，FS 小鼠海马中的 IL-1、IL-6 和 TNF-表达减少。LIR 对 FS 小鼠的脑损伤具有保护作用，通过抑制 FS 小鼠中的炎症因子来保护脑损伤。我们的发现为减轻和延缓 FS 的发展以及预防和治疗 FS 引起的脑损伤提供了参考。
