El-Demerdash Fatma M, Talaat Yousra, Ghanem Nora F, Kang Wenyi
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
Evid Based Complement Alternat Med. 2022 Apr 27;2022:5224207. doi: 10.1155/2022/5224207. eCollection 2022.
The present study evaluated the antioxidant capacity and antidiabetic effect of in diabetic rats. Rats were grouped as follows: control, aqueous extract (ADAE, 1 g/kg, daily and orally), streptozotocin (STZ, 50 mg/kg BW, single intraperitoneal dose), and STZ plus ADAE, respectively. Twenty-eight components were detected by GC-MS analysis with high phenolic contents and high DPPH scavenging activity. In vivo results revealed that rats treated with STZ showed a highly significant elevation in blood glucose and a decrease in insulin hormone levels. Thiobarbituric acid-reactive substances and hydrogen peroxide levels were elevated, while bodyweight, enzymatic, and nonenzymatic antioxidants were significantly decreased. Furthermore, histopathological and immunohistochemical insulin expression, besides ultrastructure microscopic variations (-cells, -cells, and δ-cells), were seen in pancreas sections supporting the obtained biochemical changes. Otherwise, rats supplemented with ADAE alone showed an improved antioxidant status and declined lipid peroxidation. Moreover, diabetic rats augmented with ADAE showed significant modulation in oxidative stress markers and different pancreatic tissue investigations compared to diabetic ones. Conclusively, ADAE has a potent antioxidant and hypoglycemic influence that may be utilized as a health-promoting complementary therapy in diabetes mellitus.
本研究评估了[提取物名称未给出]对糖尿病大鼠的抗氧化能力和抗糖尿病作用。大鼠分组如下：对照组、[提取物名称未给出]水提取物（ADAE，1克/千克，每日口服）、链脲佐菌素（STZ，50毫克/千克体重，单次腹腔注射剂量）以及STZ加ADAE组。通过气相色谱 - 质谱分析检测到28种成分，其具有高酚含量和高DPPH清除活性。体内实验结果显示，用STZ处理的大鼠血糖显著升高，胰岛素激素水平降低。硫代巴比妥酸反应性物质和过氧化氢水平升高，而体重、酶促和非酶促抗氧化剂显著降低。此外，在胰腺切片中观察到组织病理学和免疫组织化学胰岛素表达，以及超微结构微观变化（α细胞、β细胞和δ细胞），支持所获得的生化变化。否则，单独补充ADAE的大鼠抗氧化状态得到改善，脂质过氧化降低。此外，与糖尿病大鼠相比，补充ADAE的糖尿病大鼠在氧化应激标志物和不同胰腺组织研究方面表现出显著调节。总之，ADAE具有强大的抗氧化和降血糖作用，可作为糖尿病促进健康的辅助治疗方法。
