Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.
Oxid Med Cell Longev. 2022 Mar 19;2022:1499510. doi: 10.1155/2022/1499510. eCollection 2022.
The present research intended to assess the possible protective and hypoglycemic effect of fruit aqueous extract (ADAE) in diabetic rats. The scavenging antioxidant capabilities of ADAE were evaluated using GC-MS analysis. In addition, rats were divided into four groups: control, ADAE, streptozotocin-induced DM (STZ), and STZ-treated rats + ADAE in an in vivo investigation. GC-MS analysis of ADAE was shown to include major components with high total phenolic contents and high DPPH scavenging activity. In diabetic rats, significant elevation in blood glucose level, lipid peroxidation, bilirubin, and lactate dehydrogenase activity as well as a change in lipid profile was observed, while insulin, body and liver weights, enzymatic and nonenzymatic antioxidants, liver function biomarkers, and protein content were significantly decreased. Furthermore, changes in the expression of the peroxisome proliferator-activated receptor (PPAR-), apoptotic, and inflammation-related genes were found. In addition, histological differences in rat liver tissue architecture were discovered, corroborating the biochemical modifications. However, consuming ADAE alone reduced lipid peroxidation and improved antioxidant status. Furthermore, diabetic rats given ADAE showed significant reductions in oxidative stress indicators and biochemical parameters, as well as improved tissue structure, when compared to the diabetic rats' group. Also, ADAE supplementation protects diabetic rats' hepatic tissue by upregulating PPAR- and downregulating apoptotic and inflammatory-related gene expression. In conclusion, has beneficial protective effects so, it might be used as a complementary therapy in diabetes mellitus.
本研究旨在评估水果水提物（ADAE）在糖尿病大鼠中的可能保护和降血糖作用。采用 GC-MS 分析评估 ADAE 的清除抗氧化能力。此外，在体内研究中，将大鼠分为四组：对照组、ADAE 组、链脲佐菌素诱导的糖尿病（STZ）组和 STZ 处理大鼠+ADAE 组。GC-MS 分析表明 ADAE 包含具有高总酚含量和高 DPPH 清除活性的主要成分。在糖尿病大鼠中，观察到血糖水平、脂质过氧化、胆红素和乳酸脱氢酶活性显著升高，以及脂质谱发生变化，而胰岛素、体重和肝重、酶和非酶抗氧化剂、肝功能生物标志物和蛋白质含量显著降低。此外，还发现过氧化物酶体增殖物激活受体（PPAR-）、凋亡和炎症相关基因的表达发生变化。此外，还发现大鼠肝组织结构发生了变化，这与生化修饰相符。然而，单独食用 ADAE 可降低脂质过氧化并改善抗氧化状态。此外，与糖尿病大鼠组相比，给予 ADAE 的糖尿病大鼠的氧化应激指标和生化参数显著降低，组织结构得到改善。此外，ADAE 补充剂通过上调 PPAR-和下调凋亡和炎症相关基因的表达来保护糖尿病大鼠的肝组织。总之，具有有益的保护作用，因此可作为糖尿病的辅助治疗方法。
