Lim Hye-Sun, Jang Yumi, Park Gunhyuk
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju-si, Jeollanam-do 58245, Republic of Korea.
Evid Based Complement Alternat Med. 2022 Apr 27;2022:9116642. doi: 10.1155/2022/9116642. eCollection 2022.
Higenamine [1-[(4-hydroxyphenyl) methyl]-1, 2, 3, 4-tetrahydroisoquinoline-6, 7-diol], a potent cardiotonic compound from , contributes to vascular relaxation and bronchodilation. However, the effects and mechanisms of action of higenamine on skin aging remain poorly understood. In this study, the effects of higenamine on UVB-induced photoaging were examined in the hairless mouse model.
The dorsal skin of hairless mice (CrlOri : SKH1) was exposed to chronic UVB irradiation (100-300 mJ/cm for 6 weeks), with subsequent administration of higenamine (1-20 mg/kg, p.o.) for 2 weeks. TGF-, Smad3 DNA-binding phosphorylation, and COL1A1 levels were analyzed by immunohistochemistry, and histological analysis of the skin was performed via H&E and MT staining.
Higenamine increased TGF-, Smad3 DNA-binding phosphorylation, and COL1A1 expression in primary human fibroblast cells and mouse skin. Higenamine suppressed UVB-induced photoaging via skin recovery, improved epidermal thickness, and prevented Smad3, DNA-binding phosphorylation, and COL1A1 depletion via TGF- signaling.
Higenamine enhances collagen production in the skin through TGF-/Smad3 signaling and potentially suppresses UVB-induced skin aging.
去甲乌药碱[1-[(4-羟基苯基)甲基]-1,2,3,4-四氢异喹啉-6,7-二醇]是一种从[来源未提及]中提取的强效强心化合物，具有血管舒张和支气管扩张作用。然而，去甲乌药碱对皮肤衰老的影响及其作用机制仍知之甚少。在本研究中，我们在无毛小鼠模型中研究了去甲乌药碱对紫外线B（UVB）诱导的光老化的影响。
将无毛小鼠（CrlOri : SKH1）背部皮肤暴露于慢性UVB照射（100 - 300 mJ/cm²，持续6周），随后给予去甲乌药碱（1 - 20 mg/kg，口服）2周。通过免疫组织化学分析转化生长因子（TGF）-β、Smad3 DNA结合磷酸化和Ⅰ型胶原蛋白α1（COL1A1）水平，并通过苏木精和伊红（H&E）染色及甲苯胺蓝（MT）染色对皮肤进行组织学分析。
去甲乌药碱可增加原代人成纤维细胞和小鼠皮肤中TGF-β、Smad3 DNA结合磷酸化及COL1A1的表达。去甲乌药碱通过促进皮肤恢复抑制UVB诱导的光老化，改善表皮厚度，并通过TGF-β信号通路防止Smad3、DNA结合磷酸化及COL1A1的减少。
去甲乌药碱通过TGF-β/Smad3信号通路增强皮肤中的胶原蛋白生成，并可能抑制UVB诱导的皮肤衰老。
