Liamas Evangelos, Thomas Owen R T, Muñoz Anna Igual, Zhang Zhenyu J
School of Chemical Engineering, University of Birmingham Edgbaston Birmingham B15 2TT UK
Department of Chemical and Nuclear Engineering, Universidad Politécnica de Valencia Valencia E-46071 Spain
RSC Adv. 2019 Oct 24;9(59):34265-34273. doi: 10.1039/c9ra05988a. eCollection 2019 Oct 23.
An electrochemical quartz crystal microbalance (EQCM) was used to examine the electrochemical behaviour of pure titanium in phosphate buffered saline (PBS) and PBS-containing bovine serum albumin (BSA) solutions, and the associated adsorption characteristics of BSA under cathodic and anodic applied potentials. It was found that the electrochemical behaviours of bulk titanium substrate and titanium-coated QCM sensors are slightly different in PBS buffer solution, which is attributed to the difference in their surface roughness. The oxide film formed on the surface of the QCM sensor during potentiostatic tests was found to affect its electrochemical behaviour, while cathodic cleaning is not sufficient to have it removed. Lastly, the excessive amount of electrons on the titanium surface upon application of a cathodic potential could result in the desorption of BSA due to electrostatic repulsion and protein dehydration. In contrast, application of anodic potential charges the titanium surface positively and can facilitate protein adsorption when the surface is not saturated with protein.
采用电化学石英晶体微天平（EQCM）研究了纯钛在磷酸盐缓冲盐水（PBS）和含牛血清白蛋白（BSA）的PBS溶液中的电化学行为，以及在阴极和阳极施加电位下BSA的相关吸附特性。研究发现，块状钛基底和涂钛QCM传感器在PBS缓冲溶液中的电化学行为略有不同，这归因于它们表面粗糙度的差异。发现在恒电位测试过程中在QCM传感器表面形成的氧化膜会影响其电化学行为，而阴极清洗不足以将其去除。最后，施加阴极电位时钛表面上过量的电子可能会由于静电排斥和蛋白质脱水导致BSA解吸。相比之下，施加阳极电位会使钛表面带正电，并且当表面蛋白质不饱和时可以促进蛋白质吸附。
