Farley M M, Shafer W M, Spitznagel J K
Infect Immun. 1987 Jun;55(6):1536-9. doi: 10.1128/iai.55.6.1536-1539.1987.
A 57,000-dalton protein (CAP57) purified from human polymorphonuclear leukocytes has antimicrobial activity against a number of gram-negative bacteria. We developed a procedure using solid-phase Iodo-gen to radiolabel CAP57 without destroying its antibacterial activity. Iodinated and native CAP57 were electrophoretically identical. Autoradiographs of sodium dodecyl sulfate-polyacrylamide gels revealed greater than 95% of the 125I in a single heavy band in the 57,000-molecular-weight region. The quantity of [125I]CAP57 bound to bacterial test strains was directly proportional to the sensitivity to CAP57.
从人多形核白细胞中纯化出的一种57000道尔顿的蛋白质（CAP57）对多种革兰氏阴性菌具有抗菌活性。我们开发了一种使用固相碘苷来对CAP57进行放射性标记的方法，且不破坏其抗菌活性。碘化的CAP57和天然CAP57在电泳上是相同的。十二烷基硫酸钠 - 聚丙烯酰胺凝胶的放射自显影片显示，在57000分子量区域的单个重带中，超过95%的125I存在于此。与细菌测试菌株结合的[125I]CAP57的量与对CAP57的敏感性成正比。
