Surface phosphonation treatment shows dose-dependent enhancement of the bioactivity of polyetheretherketone.

Polyetheretherketone (PEEK) is a promising alternative for biomedical metallic implants in orthopedic and dental applications because its elastic modulus is similar to that of bone. However, PEEK is a bioinert material that cannot be integrated with host bone. Our previous study showed surface phosphonation enhanced the osteogenic activity of PEEK. The purpose of this study was to evaluate the effect of the density of phosphonate groups on the bioactivity of PEEK. X-ray photoelectron spectroscopy and water contact angle measurement confirmed the successful grafting of different densities of phosphonate groups to the PEEK surface using a one-step ultraviolet-initiated graft polymerization method. Atomic force microscopy revealed that the surface treatment did not significantly alter the surface topography and roughness. biological evaluations showed that MC3T3-E1 osteoblast responses including adhesion, spreading, proliferation, alkaline phosphatase activity, extracellular matrix mineralization, collagen secretion, and osteogenesis-related gene expression exhibited dose-dependent enhancement depending on the density of phosphonate groups. Most importantly, histological analysis and biomechanical tests showed that in a rat femur implantation model, PEEK bearing phosphonate groups had a better bone-to-implant contact ratio and corresponding bone-to-implant bonding strength at 12 weeks post-implantation than unmodified PEEK. Thus, this work provides a simple method to boost the osteogenic activity and osseointegration ability of PEEK, which has potential clinical applications in orthopedic and dental implants.