Liu Xin, Xie Jianhua, Jia Shuo, Huang Lixin, Wang Zhijun, Li Chang, Xie Mingyong
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Int J Biol Macromol. 2017 May;98:576-581. doi: 10.1016/j.ijbiomac.2017.02.028. Epub 2017 Feb 9.
Polysaccharides (CP) extracted from the leaves of Cyclocarya paliurus (C. paliurus) have been shown to possess a variety of biological activities. In present study, CP was successfully modified to obtain its acetylated derivative Ac-CP. Its potential immunomodulatory activities on RAW264.7 macrophages were investigated. Results showed that the acetylated polysaccharide Ac-CP could significantly stimulate macrophage proliferation, its actions were significantly stronger than that of the corresponding unmodified polysaccharide, CP. Meanwhile, the NO production activities of macrophages were not significantly enhanced by Ac-CP compared to CP group. In addition, both the phagocytic activity and levels of cytokines TNF-a, IL-1β and IL-6 were enhanced in the RAW264.7 macrophages by stimulation of Ac-CP. These results indicated that the acetylated derivative Ac-CP could enhance the activation of peritoneal macrophages, and acetylation modification can enhance the immunomodulation function of CP, indicating the potential application of acetylated polysaccharide as an immunotherapeutic adjuvant.
从青钱柳（Cyclocarya paliurus）叶片中提取的多糖（CP）已被证明具有多种生物活性。在本研究中，CP被成功修饰以获得其乙酰化衍生物Ac-CP。研究了其对RAW264.7巨噬细胞的潜在免疫调节活性。结果表明，乙酰化多糖Ac-CP能显著刺激巨噬细胞增殖，其作用明显强于相应的未修饰多糖CP。同时，与CP组相比，Ac-CP对巨噬细胞的NO产生活性没有显著增强。此外，通过Ac-CP刺激，RAW264.7巨噬细胞的吞噬活性以及细胞因子TNF-α、IL-1β和IL-6水平均得到增强。这些结果表明，乙酰化衍生物Ac-CP可增强腹腔巨噬细胞的活化，乙酰化修饰可增强CP的免疫调节功能，表明乙酰化多糖作为免疫治疗佐剂的潜在应用价值。
