Wicher V, Wicher K, Gruhn R F
Int Arch Allergy Appl Immunol. 1987;83(1):104-8. doi: 10.1159/000234339.
Sera of 20 male rabbits that were used as frequent donors of semen and age-matched normal male and female rabbit controls were examined by an enzyme-linked immunosorbent assay (ELISA), with sperm and seminal fluid as antigens. Five of the 20 semen donors developed an especially high humoral response to seminal fluid and spermatozoa, similar to that observed in some female breeders. The antibodies increased gradually during the 9 months of semen collection. The specificity of the antibodies was demonstrated by total or partial absorption with seminal fluid or spermatozoa. The antibodies were virtually all IgM and were directed against the acrosomal cap region of the spermatozoa, but variable fluorescence was also observed in the postnuclear region and tail.
以精子和精液为抗原，通过酶联免疫吸附测定（ELISA）对20只用作频繁精液供体的雄性兔以及年龄匹配的正常雄性和雌性兔对照的血清进行检测。20只精液供体中有5只对精液和精子产生了特别高的体液免疫反应，类似于在一些雌性繁殖兔中观察到的情况。在9个月的采精期间，抗体逐渐增加。通过用精液或精子进行全部或部分吸收来证明抗体的特异性。这些抗体几乎都是IgM，且针对精子的顶体帽区域，但在核后区域和尾部也观察到了可变荧光。
