Pfeifhofer-Obermair Christa, Brigo Natascha, Tymoszuk Piotr, Weiss And Günter
Department of Internal Medicine II, Medical University of Innsbruck, Innsbruck, Austria.
Data Analytics As a Service Tirol.
Bio Protoc. 2022 Apr 5;12(7):e4378. doi: 10.21769/BioProtoc.4378.
serovar Typhimurium Typhimurium) is a Gram-negative, facultative intracellular bacterium, which causes gastrointestinal disorders in humans, and systemic, typhoid fever-like infections in mice. Our current knowledge regarding the involvement of cellular and humoral immunity in the defense from Typhimurium infections is largely based on animal models with attenuated strains. Cells of the innate immune system act as one of the first barriers in the defense from bacteria. We established a robust experimental model for the characterization of these cell types and their response during host-pathogen interactions. Therefore, this protocol focuses on the characterization of macrophages, monocytes, and neutrophils in the spleens of infected animals by employing multi-color flow cytometry.
鼠伤寒血清型（鼠伤寒沙门氏菌）是一种革兰氏阴性兼性胞内细菌，可导致人类胃肠道疾病，并在小鼠中引发全身性伤寒热样感染。我们目前关于细胞免疫和体液免疫在抵御鼠伤寒感染中的作用的认识，很大程度上基于使用减毒株的动物模型。先天免疫系统的细胞是抵御细菌的第一道防线之一。我们建立了一个强大的实验模型，用于表征这些细胞类型及其在宿主-病原体相互作用中的反应。因此，本方案重点介绍通过多色流式细胞术对感染动物脾脏中的巨噬细胞、单核细胞和中性粒细胞进行表征。
