脓毒症和脓毒性休克动物模型的优化

Refinement of animal models of sepsis and septic shock.

作者信息

Lilley Elliot, Armstrong Rachel, Clark Nicole, Gray Peter, Hawkins Penny, Mason Karen, López-Salesansky Noelia, Stark Anne-Katrien, Jackson Simon K, Thiemermann Christoph, Nandi Manasi

机构信息

*Research Animals Department, RSPCA, Southwater; †Huntington Life Sciences, Huntington; ‡University College London, London; §Animals in Science Regulation Unit, Home Office, London; ∥Royal Veterinary College, London; ¶The Babraham Institute, Cambridge; **Centre for Biomedical Research, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth; ††The William Harvey Research Institute, Queen Mary University of London, London; and ‡‡Institute of Pharmaceutical Science, Kings College London, London, United Kingdom.

出版信息

Shock. 2015 Apr;43(4):304-16. doi: 10.1097/SHK.0000000000000318.

DOI:10.1097/SHK.0000000000000318

PMID:25565638

Abstract

This report aims to facilitate the implementation of the Three Rs (replacement, reduction, and refinement) in the use of animal models or procedures involving sepsis and septic shock, an area where there is the potential of high levels of suffering for animals. The emphasis is on refinement because this has the greatest potential for immediate implementation. Specific welfare issues are identified and discussed, and practical measures are proposed to reduce animal use and suffering as well as reducing experimental variability and increasing translatability. The report is based on discussions and submissions from a nonregulatory expert working group consisting of veterinarians, animal technologists, and scientists with expert knowledge relevant to the field.

摘要

本报告旨在推动在涉及脓毒症和脓毒性休克的动物模型使用或实验程序中实施“3R原则”（替代、减少和优化），在这一领域动物可能遭受极大痛苦。重点在于优化，因为其具有最直接的实施潜力。报告识别并讨论了具体的福利问题，提出了切实可行的措施，以减少动物使用和痛苦，同时降低实验变异性并提高可转化性。本报告基于一个非监管专家工作组的讨论和意见提交，该工作组由兽医、动物技术人员以及在该领域拥有专业知识的科学家组成。