Xie Wenyuan, Wang Honglei, Tong Yen Wah, Sankarakumar Niranjani, Yin Ming, Wu Defeng, Duan Xiaoli
Institute for Innovative Materials and Energy, Yangzhou University Yangzhou 225002 Jiangsu People's Republic of China
School of Chemistry and Chemical Engineering, Yangzhou University Yangzhou 225002 Jiangsu People's Republic of China.
RSC Adv. 2019 Jul 29;9(41):23425-23434. doi: 10.1039/c9ra02805f.
A surface imprinting method is presented herein for the development of a highly selective yet highly permeable molecularly imprinted membrane for protein separation and purification. The resultant protein imprinted membrane was shown to exhibit great potential for the efficient separation of the template protein from a binary mixture and a cell lysate solution, while maintaining high transport flux for complementary molecules. Bovine Serum Albumin (BSA) and Lysozyme (Lys) were individually immobilized on a cellulose acetate membrane as template molecules. surface crosslinking polymerization was then used for protein imprinting on the membrane for a controlled duration. Both membranes showed high adsorption capacity towards template proteins in the competitive batch rebinding tests. In addition, the adsorption capacity could be greatly enhanced in a continuous permeation procedure, where the resultant membrane specifically adsorbed the template protein for more than 40 h. Moreover, this is the first report of purification of a specific protein from the cell broth mixture using a molecularly imprinted membrane. The protein imprinted membrane enables the transport of multiple non-template proteins with high permeation rate in a complex system, thus opening the way for high efficiency protein separation at a low cost for the industry.
本文介绍了一种表面印迹方法，用于开发一种高选择性且高渗透性的分子印迹膜，用于蛋白质的分离和纯化。所得的蛋白质印迹膜显示出从二元混合物和细胞裂解液中有效分离模板蛋白的巨大潜力，同时保持对互补分子的高传输通量。牛血清白蛋白（BSA）和溶菌酶（Lys）分别作为模板分子固定在醋酸纤维素膜上。然后使用表面交联聚合在膜上进行蛋白质印迹，持续时间可控。在竞争性批量再结合试验中，两种膜对模板蛋白均显示出高吸附容量。此外，在连续渗透过程中，吸附容量可大大提高，所得膜在40多个小时内特异性吸附模板蛋白。而且，这是首次使用分子印迹膜从细胞培养液混合物中纯化特定蛋白质的报道。蛋白质印迹膜能够在复杂系统中以高渗透速率传输多种非模板蛋白，从而为工业上低成本高效蛋白质分离开辟了道路。
