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利用提取物绿色合成银纳米颗粒及其对草莓贮藏期间微生物腐烂的防护作用。

Green synthesis of silver nanoparticles with extract and its protection against microbial decay of strawberries during storage.

作者信息

Oliveira Shirlene Souza, Braga Gilberto Costa, Cordeiro Noélle Khristinne, Stangarlin José Renato, Alves Helton José

机构信息

Center of Agricultural Sciences, State University of Western Paraná-UNIOESTE, Marechal Cândido Rondon, Paraná 85960-000 Brasil.

Scanning Electron Microscopy Laboratory, Federal University of Paraná-UFPR, Palotina, Paraná 85950-000 Brasil.

出版信息

J Food Sci Technol. 2022 May;59(5):2025-2034. doi: 10.1007/s13197-021-05217-y. Epub 2021 Aug 6.

Abstract

Silver nanoparticles (AgNPs) can be produced through an easy and safe process called green synthesis and have been considered an efficient antimicrobial agent. The antimicrobial effect of silver nanoparticles green synthesized with (aveloz) can be a promising technique for preserving stored strawberries. The objective of this work was to perform a green synthesis of AgNPs with aveloz extract (Av) and evaluate its effect on the physiology and preservation of stored strawberries. Silver nitrate was reduced with Av to produce Av-AgNPs. The Av-AgNPs were characterized by Scanning Electron Microscope, Energy Dispersive X-ray Spectrometry, and laser diffraction. The in vitro antifungal activity of Av-AgNPs was evaluated against and . Strawberries were treated with Av-AgNPs and stored (5 °C) for 12 days. Respiratory rate, decay, fresh mass loss, firmness, total phenolics and antioxidant activity of the strawberries were evaluated. According to the results, Av-AgNPs synthesis was performed, and it presented sizes between 40 and 90 nm. Av-AgNPs inhibited but was less effective for . Total phenolic compounds, antioxidant activity, fresh mass loss and firmness of strawberries were not influenced by Av-AgNPs. Treated strawberries had a lower respiratory rate than the control and showed no symptoms of microbiological deterioration until 9 days of storage, while in the control the deterioration symptoms started after 3 days. This study showed that the green synthesis of AgNPs with Av produced nanoparticles smaller than 100 nm, and that they were effective against strawberries decay during storage, indicating to be a promising protection technique against decay.

摘要

银纳米颗粒(AgNPs)可以通过一种名为绿色合成的简单且安全的过程来制备,并且已被视为一种高效的抗菌剂。用(阿韦洛)绿色合成的银纳米颗粒的抗菌效果可能是一种保存储存草莓的有前景的技术。这项工作的目的是用阿韦洛提取物(Av)进行AgNPs的绿色合成,并评估其对储存草莓的生理和保存效果的影响。用Av还原硝酸银以制备Av-AgNPs。通过扫描电子显微镜、能量色散X射线光谱仪和激光衍射对Av-AgNPs进行表征。评估了Av-AgNPs对和的体外抗真菌活性。用Av-AgNPs处理草莓并在5℃下储存12天。评估了草莓的呼吸速率、腐烂情况、鲜重损失、硬度、总酚含量和抗氧化活性。根据结果,进行了Av-AgNPs的合成,其尺寸在40至90纳米之间。Av-AgNPs抑制了,但对的效果较差。草莓的总酚化合物、抗氧化活性、鲜重损失和硬度不受Av-AgNPs的影响。处理过的草莓呼吸速率低于对照,并且在储存9天之前没有出现微生物变质的症状,而对照在3天后就开始出现变质症状。这项研究表明,用Av绿色合成AgNPs产生的纳米颗粒小于100纳米,并且它们在储存期间对草莓腐烂有效,表明是一种有前景的防止腐烂的保护技术。

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