银纳米颗粒对阿萨希毛孢子菌的抗真菌作用。
The antifungal effect of silver nanoparticles on Trichosporon asahii.
作者信息
Xia Zhi-Kuan, Ma Qiu-Hua, Li Shu-Yi, Zhang De-Quan, Cong Lin, Tian Yan-Li, Yang Rong-Ya
机构信息
Department of Dermatology, General Hospital of Beijing Military Command of PLA, Nanmencang, Dongcheng District, Beijing, PR China; Third Military Medical University, Gaotanyanzhengjie, Shapingba District, Chongqing, PR China.
Department of Dermatology, General Hospital of Beijing Military Command of PLA, Nanmencang, Dongcheng District, Beijing, PR China.
出版信息
J Microbiol Immunol Infect. 2016 Apr;49(2):182-8. doi: 10.1016/j.jmii.2014.04.013. Epub 2014 May 28.
BACKGROUND/PURPOSE: Silver nanoparticles are receiving increasing attention in biomedical applications. This study aims at evaluating the antifungal properties of silver nanoparticles against the pathogenic fungus Trichosporon asahii.
METHODS
The growth of T. asahii on potato dextrose agar medium containing different concentrations of silver nanoparticles was examined and the antifungal effect was evaluated using minimum inhibitory concentration. Scanning and transmission electron microscopy were also used to investigate the antifungal effect of silver nanoparticles on T. asahii.
RESULTS
Silver nanoparticles had a significant inhibitory effect on the growth of T. asahii. The minimum inhibitory concentration of silver nanoparticles against T. asahii was 0.5 μg/mL, which was lower than amphotericin B, 5-flucytosine, caspofungin, terbinafine, fluconazole, and itraconazole and higher than voriconazole. Silver nanoparticles obviously damaged the cell wall, cell membrane, mitochondria, chromatin, and ribosome.
CONCLUSION
Our results demonstrate that silver nanoparticles have good antifungal activity against T. asahii. Based on our electron microscopy observations, silver nanoparticles may inhibit the growth of T. asahii by permeating the fungal cell and damaging the cell wall and cellular components.
背景/目的：银纳米颗粒在生物医学应用中受到越来越多的关注。本研究旨在评估银纳米颗粒对致病性真菌阿萨希毛孢子菌的抗真菌特性。
方法
检测阿萨希毛孢子菌在含有不同浓度银纳米颗粒的马铃薯葡萄糖琼脂培养基上的生长情况，并使用最低抑菌浓度评估抗真菌效果。还利用扫描电子显微镜和透射电子显微镜研究银纳米颗粒对阿萨希毛孢子菌的抗真菌作用。
结果
银纳米颗粒对阿萨希毛孢子菌的生长具有显著抑制作用。银纳米颗粒对阿萨希毛孢子菌的最低抑菌浓度为0.5μg/mL，低于两性霉素B、5-氟胞嘧啶、卡泊芬净、特比萘芬、氟康唑和伊曲康唑，高于伏立康唑。银纳米颗粒明显破坏了细胞壁、细胞膜、线粒体、染色质和核糖体。
结论
我们的结果表明，银纳米颗粒对阿萨希毛孢子菌具有良好的抗真菌活性。基于我们的电子显微镜观察结果，银纳米颗粒可能通过穿透真菌细胞并破坏细胞壁和细胞成分来抑制阿萨希毛孢子菌的生长。
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