Johann S, Hinton S M
J Bacteriol. 1987 May;169(5):1911-6. doi: 10.1128/jb.169.5.1911-1916.1987.
The nucleotide sequence of a Sau3A1 restriction nuclease fragment that complemented an Escherichia coli chlD::Mu cts mutant strain was determined. DNA and deduced amino acid sequence analysis revealed two open reading frames (ORFs) that potentially codes for proteins with amino acid sequence homology with binding protein-dependent transport systems. One of the ORFs showed a sequence that encoded a protein with properties that were characteristic of a hydrophobic inner membrane protein. The other ORF, which was responsible for complementing a chlD mutant, encoded a protein with conserved sequences in nucleotide-binding proteins and hydrophilic inner membrane proteins in active transport systems. A proposal that the chlD locus is the molybdate transport operon is discussed in terms of the chlD phenotype.
测定了一个能互补大肠杆菌chlD::Mu cts突变菌株的Sau3A1限制性核酸内切酶片段的核苷酸序列。DNA和推导的氨基酸序列分析揭示了两个开放阅读框（ORF），它们可能编码与依赖结合蛋白的转运系统具有氨基酸序列同源性的蛋白质。其中一个ORF显示出编码一种具有疏水内膜蛋白特征性质的蛋白质的序列。另一个负责互补chlD突变体的ORF编码一种在主动运输系统的核苷酸结合蛋白和亲水内膜蛋白中具有保守序列的蛋白质。根据chlD表型讨论了chlD基因座是钼酸盐转运操纵子的提议。
