Kärst U, Suetin S, Friedrich C G
J Bacteriol. 1987 May;169(5):2079-85. doi: 10.1128/jb.169.5.2079-2085.1987.
In Alcaligenes eutrophus, the formation of the hydrogenases and of five new peptides is subject to the hydrogenase control system. Of these, the B peptide was purified to homogeneity. This protein (Mr, 37,500) was composed of two identical subunits (Mr, 18,800). Antibodies against the B protein were used for its quantification by rocket immunoelectrophoresis. About 4% of the total protein consisted of the B protein; its molar ratio to the NAD-linked hydrogenase was about 4:1. The B protein appeared to be associated with the NAD-linked hydrogenase, as shown by gel filtration analysis with Sephadex G-200. The B protein was not detected in cells that had not expressed the hydrogenase proteins or that lacked the genetic information of the hydrogen-oxidizing character; it was also not detected in Tn5 insertional mutants that were unable to form soluble hydrogenase antigens. Immunochemical analysis of other species and genera than A. eutrophus revealed that only strains able to form a NAD-linked hydrogenase also formed B-protein antigens. The B protein is not required for the catalytic activity of soluble hydrogenase in vitro; its function is at present unknown.
在真养产碱菌中，氢化酶和五种新肽的形成受氢化酶控制系统的调控。其中，B肽被纯化至同质。该蛋白质（分子量为37,500）由两个相同的亚基（分子量为18,800）组成。针对B蛋白的抗体用于通过火箭免疫电泳对其进行定量。总蛋白中约4%由B蛋白组成；其与NAD连接的氢化酶的摩尔比约为4:1。如用葡聚糖凝胶G-200进行的凝胶过滤分析所示，B蛋白似乎与NAD连接的氢化酶相关。在未表达氢化酶蛋白或缺乏氢氧化特性遗传信息的细胞中未检测到B蛋白；在无法形成可溶性氢化酶抗原的Tn5插入突变体中也未检测到。对除真养产碱菌之外的其他物种和属的免疫化学分析表明，只有能够形成NAD连接的氢化酶的菌株也形成B蛋白抗原。B蛋白在体外对可溶性氢化酶的催化活性不是必需的；其功能目前尚不清楚。
