Peguero Ricardo L, Bimbo-Szuhai Andras, Massaro Ian T, Roach Kevin D, Corbo Christopher P
Laboratory for Developmental Brain Research and Neuroplasticity, Department of Biological Sciences, Wagner College, Staten Island, New York, USA.
Microsc Res Tech. 2022 Aug;85(8):2924-2930. doi: 10.1002/jemt.24142. Epub 2022 May 9.
Biological 3D models have a multitude of applications in both research and academic settings, however the generation of such models at an ultrastructural scale has remained a daunting task. Here our group presents a method by which ultrastructural 3D models can be generated using tandem scanning electron microscopy (SEM) and photogrammetry. Our methods include a novel technique for mounting specimens for SEM which allowed our group to capture images from all angles around the specimen. Our results demonstrate that using our technique is adequate for the construction of an interactive, ultrastructural 3D model that can be viewed from all orientations. We ultimately see use for these models in educational settings and research when the 3D analysis of ultrastructural anatomy is necessary. HIGHLIGHTS: Scanning electron microscopy (SEM) was used in tandem with photogrammetry to generate an ultrastructural three-dimensional model of a whole zebrafish brain. A novel mounting technique for samples in SEM was used to view the sample from all angles. This technique can be applied to a wide range of samples for 3D model generation for use in several settings, including research and academia.
生物三维模型在研究和学术环境中都有多种应用，然而，在超微结构尺度上生成此类模型仍然是一项艰巨的任务。在此，我们团队提出了一种利用串联扫描电子显微镜（SEM）和摄影测量法生成超微结构三维模型的方法。我们的方法包括一种用于为SEM安装标本的新技术，该技术使我们团队能够从标本周围的各个角度捕获图像。我们的结果表明，使用我们的技术足以构建一个可以从所有方向查看的交互式超微结构三维模型。当需要对超微结构解剖进行三维分析时，我们最终认为这些模型可用于教育环境和研究中。要点：扫描电子显微镜（SEM）与摄影测量法联用，生成了完整斑马鱼脑的超微结构三维模型。在SEM中使用了一种用于样品的新型安装技术，以便从各个角度观察样品。该技术可应用于广泛的样品，用于生成三维模型，以用于多种环境，包括研究和学术界。
