Stevenson S
J Bone Joint Surg Am. 1987 Apr;69(4):573-82.
Dog leukocyte antigen (DLA)-matched and mismatched, fresh and frozen (cryopreserved) osteochondral allografts of the proximal part of the radius were implanted orthotopically in beagles. The systemic and local (intra-articular) immune responses were monitored for eleven months using a 51chromium release assay with donor peripheral-blood lymphocytes as target cells. DLA-mismatched fresh grafts markedly and persistently stimulated the formation of antibody directed against donor cell-surface antigens, while DLA-matched grafts did not elicit systemically detectable antibody. The same general pattern was noted when antibody-dependent cell-mediated cytotoxicity was assayed. There was measurable cell-mediated immunity to donor cells from six weeks after surgery onward, although no distinct pattern or differences between experimental groups were noted. Higher titers of anti-DLA antibody were found in synovial fluid than in serum; in fact, synovial antibody was detectable when systemic antibody was not. Joints that received DLA-mismatched grafts had the highest titer of antibody and the intra-articular response was significantly reduced when the graft had been frozen.
将与犬白细胞抗原（DLA）匹配和不匹配的新鲜及冷冻（低温保存）的桡骨近端骨软骨同种异体移植物原位植入比格犬体内。以供体外周血淋巴细胞为靶细胞，采用51铬释放试验，对全身和局部（关节内）免疫反应进行了11个月的监测。DLA不匹配的新鲜移植物显著且持续地刺激了针对供体细胞表面抗原的抗体形成，而DLA匹配的移植物未引发可在全身检测到的抗体。在检测抗体依赖性细胞介导的细胞毒性时也观察到了相同的总体模式。术后六周起对供体细胞有可测量的细胞介导免疫，尽管未观察到实验组之间有明显模式或差异。滑液中抗DLA抗体的滴度高于血清；事实上，当全身抗体不可检测时，滑液中的抗体仍可检测到。接受DLA不匹配移植物的关节抗体滴度最高，当移植物冷冻时，关节内反应显著降低。
